Innovative chemical tools to address analytical challenges of protein phosphorylation and glycosylation

M Li, Y Xiong, G Qing - Accounts of Chemical Research, 2023 - ACS Publications
Conspectus Protein post-translational modifications (PTMs) modulate almost all cellular
processes. Aberrant PTMs are closely bound to various human diseases, which greatly …

Nanopore: Emerging for detecting protein post-translational modifications

X Zhao, H Qin, M Tang, X Zhang, G Qing - TrAC Trends in Analytical …, 2024 - Elsevier
Protein post-translational modifications (PTMs) play pivotal roles in almost every cellular
process, influencing protein stability, localization, interactions, and enzymatic activities. PTM …

Direct identification of complex glycans via a highly sensitive engineered nanopore

G Yao, Y Tian, W Ke, J Fang, S Ma, T Li… - Journal of the …, 2024 - ACS Publications
The crucial roles that glycans play in biological systems are determined by their structures.
However, the analysis of glycan structures still has numerous bottlenecks due to their …

Enrichment driven glycoproteomics: New materials, new methods, and beyond

Y Xiong, M Li, Y Liu, X Liang, G Qing - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Glycoproteomics is a rapidly growing field that aims to identify and characterize protein
glycosylation events at a system level. However, the analysis of protein glycosylation in …

Sensing PEGylated Peptide Conformations Using a Protein Nanopore

R Satheesan, D Vikraman, P Jayan, V Vijayan… - Nano Letters, 2024 - ACS Publications
Membrane pores are exploited for the stochastic sensing of various analytes, and here, we
use electrical recordings to explore the interaction of PEGylated peptides of different sizes …

Towards chemoenzymatic labeling strategies for profiling protein glycosylation

Y Tian, S Ma, L Wen - Current Opinion in Chemical Biology, 2024 - Elsevier
Protein glycosylation is one of the most common and important post-translational
modifications of proteins involved in regulating glycoprotein functions. The chemoenzymatic …

Precise Capture and Dynamic Release of Circulating Liver Cancer Cells with Dual‐Histidine‐Based Cell Imprinted Hydrogels

W Sun, X You, X Zhao, X Zhang, C Yang… - Advanced …, 2024 - Wiley Online Library
Circulating tumor cells (CTCs) detection presents significant advantages in diagnosing liver
cancer due to its noninvasiveness, real‐time monitoring, and dynamic tracking. However …

[HTML][HTML] Sensitive and specific detection of saccharide species based on fluorescence: update from 2016

X Huang, Y Han, J Li, M Tang, G Qing - Analytical and Bioanalytical …, 2023 - Springer
Increasing evidence supports the critical role of saccharides in various pathophysiological
steps of tumor progression, where they regulate tumor proliferation, invasion, hematogenic …

Recent advances of nanopore-based analysis for carbohydrate and glycoconjugate

Y Zhao, Z Su, X Zhang, D Wu, Y Wu, G Li - Analytical Methods, 2024 - pubs.rsc.org
Saccharides are not only the basic constituents and nutrients of living organisms, but also
participate in various life activities, and play important roles in cell recognition, immune …

Precise Structural Analysis of Neutral Glycans Using Aerolysin Mutant T240R Nanopore

W Lu, X Zhao, M Li, Y Li, C Zhang, Y Xiong, J Li… - ACS …, 2024 - ACS Publications
Glycans play vital roles in nearly all life processes of multicellular organisms, and
understanding these activities is inseparable from elucidating the biological significance of …