In addition to the canonical right-handed double helix, other DNA structures, termed 'non-B
DNA', can form in the genomes across the tree of life. Non-B DNA regulates multiple cellular …

Repeated DNA makes up a large fraction of a typical mammalian genome, and some
repetitive elements are able to move within the genome (transposons and retrotransposons) …

Infusions of antigen-specific T cells have yielded therapeutic responses in patients with
pathogens and tumors. To broaden the clinical application of adoptive immunotherapy …

Transposons are mobile genetic elements that can be used to integrate transgenes into host
cell genomes. The piggyBac transposon system has been used for transgenesis of insects …

Viruses and transposons are efficient tools for permanently delivering foreign DNA into
vertebrate genomes but exhibit diminished activity when cargo exceeds 8 kilobases (kb) …

Transposon-based gene vectors have become indispensable tools in vertebrate genetics for
applications ranging from insertional mutagenesis and transgenesis in model species to …

Genetic modification of clinical-grade T cells is undertaken to augment function, including
redirecting specificity for desired antigen. We and others have introduced a chimeric antigen …

The stable introduction of therapeutic transgenes into human cells can be accomplished
using viral and nonviral approaches. Transduction with clinical-grade recombinant viruses …

DNA sequences from retroviruses, retrotransposons, DNA transposons, and parvoviruses
can all become integrated into the human genome. Accumulation of such sequences …

Transposable elements can be viewed as natural DNA transfer vehicles that, similar to
integrating viruses, are capable of efficient genomic insertion. The mobility of class II …