Metabolic engineering strategies to enable microbial utilization of C1 feedstocks

W Jiang, D Hernández Villamor, H Peng… - Nature Chemical …, 2021 - nature.com
Abstract One-carbon (C1) substrates are preferred feedstocks for the biomanufacturing
industry and have recently gained attention owing to their natural abundance, low …

A hybrid inorganic–biological artificial photosynthesis system for energy-efficient food production

EC Hann, S Overa, M Harland-Dunaway, AF Narvaez… - Nature food, 2022 - nature.com
Artificial photosynthesis systems are proposed as an efficient alternative route to capture
CO2 to produce additional food for growing global demand. Here a two-step CO2 …

Recent advances toward the bioconversion of methane and methanol in synthetic methylotrophs

GJ Gregory, RK Bennett, ET Papoutsakis - Metabolic Engineering, 2022 - Elsevier
Abundant natural gas reserves, along with increased biogas production, have prompted
recent interest in harnessing methane as an industrial feedstock for the production of liquid …

Growth of E. coli on formate and methanol via the reductive glycine pathway

S Kim, SN Lindner, S Aslan, O Yishai, S Wenk… - Nature chemical …, 2020 - nature.com
Engineering a biotechnological microorganism for growth on one-carbon intermediates,
produced from the abiotic activation of CO2, is a key synthetic biology step towards the …

Converting Escherichia coli to a synthetic methylotroph growing solely on methanol

FYH Chen, HW Jung, CY Tsuei, JC Liao - Cell, 2020 - cell.com
Methanol, being electron rich and derivable from methane or CO 2, is a potentially
renewable one-carbon (C1) feedstock for microorganisms. Although the ribulose …

Generation of an Escherichia coli strain growing on methanol via the ribulose monophosphate cycle

P Keller, MA Reiter, P Kiefer, T Gassler… - Nature …, 2022 - nature.com
Methanol is a liquid with high energy storage capacity that holds promise as an alternative
substrate to replace sugars in the biotechnology industry. It can be produced from CO2 or …

Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph

C Zhan, X Li, G Lan, EEK Baidoo, Y Yang, Y Liu… - Nature Catalysis, 2023 - nature.com
Methanol, an organic one-carbon (C1) compound, represents an attractive alternative
carbon source for microbial fermentation. Despite considerable advancements in methanol …

Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae

MI Espinosa, RA Gonzalez-Garcia, K Valgepea… - Nature …, 2020 - nature.com
Utilising one-carbon substrates such as carbon dioxide, methane, and methanol is vital to
address the current climate crisis. Methylotrophic metabolism enables growth and energy …

A modified serine cycle in Escherichia coli coverts methanol and CO2 to two-carbon compounds

H Yu, JC Liao - Nature Communications, 2018 - nature.com
Microbial utilization of renewable one-carbon compounds, such as methane, methanol,
formic acid, and CO2, has emerged as a potential approach to increase the range of carbon …

Construction of a synthetic metabolic pathway for biosynthesis of 2, 4-dihydroxybutyric acid from ethylene glycol

CJR Frazão, N Wagner, K Rabe, T Walther - Nature Communications, 2023 - nature.com
Ethylene glycol is an attractive two-carbon alcohol substrate for biochemical product
synthesis as it can be derived from CO2 or syngas at no sacrifice to human food stocks …