Identification of two archaeal GDGT lipid–modifying proteins reveals diverse microbes capable of GMGT biosynthesis and modification

AA Garcia, GL Chadwick, XL Liu… - Proceedings of the …, 2024 - National Acad Sciences
Archaea produce unique membrane-spanning lipids (MSLs), termed glycerol dialkyl glycerol
tetraethers (GDGTs), which aid in adaptive responses to various environmental challenges …

Intein splicing efficiency and RadA levels can control the mode of archaeal DNA replication

GLS Liman, CW Lennon, JL Mandley, AM Galyon… - Science …, 2024 - science.org
Inteins (intervening proteins), mobile genetic elements removed through protein splicing,
often interrupt proteins required for DNA replication, recombination, and repair. An …

Histones direct site-specific CRISPR spacer acquisition in model archaeon

EA Watts, SC Garrett, RJ Catchpole, LM Clark… - Nature …, 2023 - nature.com
CRISPR–Cas systems provide heritable immunity against viruses and other mobile genetic
elements by incorporating fragments of invader DNA into the host CRISPR array as spacers …

Tetraether archaeal lipids promote long‐term survival in extreme conditions

GLS Liman, AA Garcia, KA Fluke… - Molecular …, 2024 - Wiley Online Library
The sole unifying feature of the incredibly diverse Archaea is their isoprenoid‐based ether‐
linked lipid membranes. Unique lipid membrane composition, including an abundance of …

Thermococcus kodakarensis TK0353 is a novel AP lyase with a new fold

PJ Caffrey, BE Eckenroth, BW Burkhart… - Journal of Biological …, 2024 - ASBMB
Hyperthermophilic organisms thrive in extreme environments prone to high levels of DNA
damage. Growth at high temperature stimulates DNA base hydrolysis resulting in …