Many research questions in fields such as personalized medicine, drug screens or systems
biology depend on obtaining consistent and quantitatively accurate proteomics data from …

This review provides a brief overview of the development of data‐independent acquisition
(DIA) mass spectrometry‐based proteomics and selected DIA data analysis tools. Various …

In mass-spectrometry-based proteomics, the identification and quantification of peptides and
proteins heavily rely on sequence database searching or spectral library matching. The lack …

Data independent acquisition (DIA) mass spectrometry is a powerful technique that is
improving the reproducibility and throughput of proteomics studies. Here, we introduce an …

Data-independent acquisition mass spectrometry (DIA-MS) is a next generation proteomic
methodology that generates permanent digital proteome maps offering highly reproducible …

The data‐independent acquisition mass spectrometry (DIA‐MS) has rapidly evolved as a
powerful alternative for highly reproducible proteome profiling with a unique strength of …

Tandem mass spectral library search (MS/MS) is the fastest way to correctly annotate MS/MS
spectra from screening small molecules in fields such as environmental analysis, drug …

Data-independent acquisition approaches typically rely on experiment-specific spectrum
libraries, requiring offline fractionation and tens to hundreds of injections. We demonstrate a …

Peptide fragmentation spectra are routinely predicted in the interpretation of mass-
spectrometry-based proteomics data. However, the generation of fragment ions has not …

In this Perspective, we discuss developments in mass-spectrometry-based proteomic
technology over the past decade from the viewpoint of our laboratory. We also reflect on …