Prospects for carbon-negative biomanufacturing

CD Scown - Trends in biotechnology, 2022 - cell.com
Biomanufacturing has the potential to reduce demand for petrochemicals and mitigate
climate change. Recent studies have also suggested that some of these products can be net …

Recent progress in the synthesis of advanced biofuel and bioproducts

BF Pfleger, R Takors - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights●Why biofuels and bioproducts remain critical to society●What molecular
products can be made by biotechnology using available feedstocks●How new systems and …

An automated cell-free workflow for transcription factor engineering

HM Ekas, B Wang, AD Silverman, JB Lucks… - ACS Synthetic …, 2024 - ACS Publications
The design and optimization of metabolic pathways, genetic systems, and engineered
proteins rely on high-throughput assays to streamline design-build-test-learn cycles …

Cell-free enzyme cascades—application and transition from development to industrial implementation

M Teshima, VP Willers, V Sieber - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights•State-of-the-art in vitro enzyme cascades to assimilate CO 2-derived C1
compounds.•Current transitioning state of the biomass-based production of commodity …

Phylogenomics and genetic analysis of solvent-producing Clostridium species

RO Jensen, F Schulz, S Roux, DM Klingeman… - Scientific data, 2024 - nature.com
The genus Clostridium is a large and diverse group within the Bacillota (formerly Firmicutes),
whose members can encode useful complex traits such as solvent production, gas …

Enzyme‐Polymer‐Conjugate‐Based Pickering Emulsions for Cell‐Free Expression and Cascade Biotransformation

H Lu, J Ouyang, WQ Liu, C Wu… - Angewandte Chemie …, 2023 - Wiley Online Library
In this study, we addressed the limitations of conventional enzyme‐polymer‐conjugate‐
based Pickering emulsions for interfacial biocatalysis, which traditionally suffer from …

Physical laboratory automation in synthetic biology

A Stephenson, L Lastra, B Nguyen, YJ Chen… - ACS Synthetic …, 2023 - ACS Publications
Synthetic Biology has overcome many of the early challenges facing the field and is entering
a systems era characterized by adoption of Design-Build-Test-Learn (DBTL) approaches …

[HTML][HTML] Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities

S Wan, M Lai, X Gao, M Zhou, S Yang, Q Li, F Li… - Synthetic and Systems …, 2024 - Elsevier
Excessive mining and utilization fossil fuels has led to drastic environmental consequences,
which will contribute to global warming and cause further climate change with severe …

Establishing a versatile toolkit of flux enhanced strains and cell extracts for pathway prototyping

X Yi, BJ Rasor, N Boadi, K Louie, TR Northen… - Metabolic …, 2023 - Elsevier
Building and optimizing biosynthetic pathways in engineered cells holds promise to address
societal needs in energy, materials, and medicine, but it is often time-consuming. Cell-free …

Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO

JC Dykstra, J van Oort, AT Yazdi, E Vossen… - Microbial Cell …, 2022 - Springer
Background Ethyl acetate is a bulk chemical traditionally produced via energy intensive
chemical esterification. Microbial production of this compound offers promise as a more …