The secretion of biotechnologically or pharmaceutically relevant recombinant proteins into
the culture supernatant of a bacterial expression host greatly facilitates their downstream …

Abstract Cutinases (EC 3.1. 1.74) belong to the α/β-hydrolase superfamily. They were
initially discovered because they are secreted by fungi to hydrolyze the ester bonds of the …

Poly (ethylene terephthalate)(PET) is one of the most commonly used plastics worldwide
and its accumulation in the environment is a global problem. PETase from Ideonella …

The biotechnology industry has become a key element in modern societies. Within this
industry, the production of recombinant enzymes and biopharmaceutical proteins is of major …

Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low% G+
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …

Bacillus subtilis can serve as a powerful platform for directed evolution, especially for
secretory enzymes. However, cloning and transformation of a DNA mutant library in B …

Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial
production of proteins. These strains can efficiently secrete proteins into the culture medium …

Bacillus subtilis has become a widely used microbial cell factory for the production of
recombinant proteins, especially those associated with foods and food processing. Recent …

Escherichia coli—the powerhouse for recombinant protein production—is rapidly gaining
status as a reliable and efficient host for secretory expression. An improved understanding of …

Abstract α-Amylase is an important amylolytic enzyme participating in hydrolysis of starch,
the most common carbohydrate in nature. Compared to plant and animal origins, microbial α …