The selectivity of the ubiquitin–26 S proteasome system (UPS) for a particular substrate
protein relies on the interaction between a ubiquitin-conjugating enzyme (E2, of which a cell …

Selective protein degradation by the 26S proteasome requires the covalent attachment of
several ubiquitin molecules in the form of a multiubiquitin chain. Ubiquitylation usually …

Skeletal muscle is a key tissue in human aging, which affects different muscle fiber types
unequally. We developed a highly sensitive single muscle fiber proteomics workflow to study …

The assembly of the Smad complex is critical for TGFβ signaling, yet the mechanisms that
inactivate or empower nuclear Smad complexes are less understood. By means of siRNA …

CHIP is a dimeric U box E3 ubiquitin ligase that binds Hsp90 and/or Hsp70 via its TPR-
domain, facilitating ubiquitylation of chaperone bound client proteins. We have determined …

Cell survival, tissue integrity and organismal health depend on the ability to maintain
functional protein networks even under conditions that threaten protein integrity. Protection …

Aging is attended by a progressive decline in protein homeostasis (proteostasis),
aggravating the risk for protein aggregation diseases. To understand the coordination …

The assembly and maintenance of the cardiac sarcomere, which contains the basic
contractile components of actin and myosin, are essential for cardiac function. While often …

Muscle wasting occurs when rates of protein degradation outstrip rates of protein synthesis.
Accelerated rates of protein degradation develop in atrophying muscle largely through …

Protein degradation in eukaryotes often requires the ubiquitin-selective chaperone p97 for
substrate recruitment and ubiquitin-chain assembly. However, the physiological relevance of …