The combination of fluorescent‐probe technology plus modern optical microscopes allows
investigators to monitor dynamic events in living cells with exquisite temporal and spatial …

Intracellular chloride ion concentration ([Cl–] i) homeostasis is critical for excitatory/inhibitory
balance and volume regulation in neurons. We quantitatively map spatiotemporal dendritic …

The application of time-resolved fluorescence spectroscopy (TRFS) to in vivo tissue
diagnosis requires a method for fast acquisition of fluorescence decay profiles in multiple …

Optical methods such as reflectance and fluorescence spectroscopy are being investigated
for their potential to aid cancer detection in a quantitative, minimally invasive manner …

Fluorescence lifetime imaging microscopy (FLIM) is a method for measuring fluorophore
lifetimes with microscopic spatial resolution, providing a useful tool for cell biologists to …

A hyperspectral fluorescence lifetime imaging (FLIM) instrument is developed to study
endogenous fluorophores in biological tissue as an optical biopsy tool. This instrument is …

This review summarizes the current status of development in photoluminescent probes,
multidimensional photoluminescence detection, and multivariate data analysis methods. It …

Fluorescence spectroscopy is a widely employed technique in chemical and biomedical
applications for noninvasive specimen interrogation and monitoring that can be performed …

Abstract Intracellular chloride ion ([Cl−] i) homeostasis is critical for synaptic
neurotransmission yet variations in subcellular domains are poorly understood owing to …

11.1 Introduction e goal of this chapter is to provide an overview of several well-known
fluorescence spectroscopy and imaging techniques that have been employed in clinical …