Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Determining how chromosomes are positioned and folded within the nucleus is critical to
understanding the role of chromatin topology in gene regulation. Several methods are …

INTRODUCTION The eukaryotic nucleus protects the genome and is enclosed by the two
membranes of the nuclear envelope. Nuclear pore complexes (NPCs) perforate the nuclear …

INTRODUCTION The nucleus harbors and protects the genetic information in eukaryotes. It
is surrounded by the nuclear envelope, which separates the nucleoplasm from the cytosol …

Nuclear pore complexes (NPCs) create large conduits for cargo transport between the
nucleus and cytoplasm across the nuclear envelope (NE),–. These multi-megadalton …

In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in
recent years as a powerful technique for structural studies of macromolecular complexes in …

Summary Human immunodeficiency virus (HIV-1) remains a major health threat. Viral capsid
uncoating and nuclear import of the viral genome are critical for productive infection. The …

Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

Cryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro
and inside cells. In addition to aligning individual particles, accurate registration of sample …

Integral membrane protein structure determination traditionally requires extraction from cell
membranes using detergents or polymers. Here, we describe the isolation and structure …