Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
A compendium of single extracellular vesicle flow cytometry
JA Welsh, GJA Arkesteijn, M Bremer… - Journal of …, 2023 - Wiley Online Library
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …
High-speed fluorescence image–enabled cell sorting
Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …
remains a technical challenge. Here, we address this by establishing high-speed image …
Clonal expansion and epigenetic inheritance of long-lasting NK cell memory
Clonal expansion of cells with somatically diversified receptors and their long-term
maintenance as memory cells is a hallmark of adaptive immunity. Here, we studied …
maintenance as memory cells is a hallmark of adaptive immunity. Here, we studied …
Marked T cell activation, senescence, exhaustion and skewing towards TH17 in patients with COVID-19 pneumonia
S De Biasi, M Meschiari, L Gibellini, C Bellinazzi… - Nature …, 2020 - nature.com
The immune system of patients infected by SARS-CoV-2 is severely impaired. Detailed
investigation of T cells and cytokine production in patients affected by COVID-19 pneumonia …
investigation of T cells and cytokine production in patients affected by COVID-19 pneumonia …
Impaired immune cell cytotoxicity in severe COVID-19 is IL-6 dependent
BACKGROUND Coronavirus disease 19 (COVID-19) is an emerging infectious disease
caused by SARS-CoV-2. Antiviral immune response is crucial to achieve pathogen …
caused by SARS-CoV-2. Antiviral immune response is crucial to achieve pathogen …
Meta-analysis of leukocyte diversity in atherosclerotic mouse aortas
The diverse leukocyte infiltrate in atherosclerotic mouse aortas was recently analyzed in 9
single-cell RNA sequencing and 2 mass cytometry studies. In a comprehensive meta …
single-cell RNA sequencing and 2 mass cytometry studies. In a comprehensive meta …
Precise reconstruction of the TME using bulk RNA-seq and a machine learning algorithm trained on artificial transcriptomes
A Zaitsev, M Chelushkin, D Dyikanov, I Cheremushkin… - Cancer Cell, 2022 - cell.com
Cellular deconvolution algorithms virtually reconstruct tissue composition by analyzing the
gene expression of complex tissues. We present the decision tree machine learning …
gene expression of complex tissues. We present the decision tree machine learning …
Immunological signature in human cases of monkeypox infection in 2022 outbreak: an observational study
Background An unprecedented global monkeypox outbreak started in May, 2022. No data
are yet available about the dynamics of the immune response against monkeypox virus. The …
are yet available about the dynamics of the immune response against monkeypox virus. The …
Extricating human tumour immune alterations from tissue inflammation
Immunotherapies have achieved remarkable successes in the treatment of cancer, but major
challenges remain,. An inherent weakness of current treatment approaches is that …
challenges remain,. An inherent weakness of current treatment approaches is that …