Spermatogonial stem cells for in vitro spermatogenesis and in vivo restoration of fertility

F Ibtisham, A Honaramooz - Cells, 2020 - mdpi.com
Spermatogonial stem cells (SSCs) are the only adult stem cells capable of passing genes
onto the next generation. SSCs also have the potential to provide important knowledge …

Gelatin—alginate—cerium oxide nanocomposite scaffold for bone regeneration

SD Purohit, H Singh, R Bhaskar, I Yadav… - Materials Science and …, 2020 - Elsevier
Worldwide the number of bone damage/fracture, due to traumatic and accidental injuries,
has been growing exponentially. Currently available treatments for bone repairing are slow …

Technologies for vitrification based cryopreservation

M Amini, JD Benson - Bioengineering, 2023 - mdpi.com
Cryopreservation is a unique and practical method to facilitate extended access to biological
materials. Because of this, cryopreservation of cells, tissues, and organs is essential to …

Evaluation of sodium alginate for encapsulation-vitrification of testicular Leydig cells

T Patra, MK Gupta - International journal of biological macromolecules, 2020 - Elsevier
This study reports encapsulation-vitrification of Leydig cells. The Leydig cells were
encapsulated in sodium alginate beads of different sizes and cryopreserved by vitrification …

Strategies for cryopreservation of testicular cells and tissues in cancer and genetic diseases

T Patra, D Pathak, MK Gupta - Cell and tissue research, 2021 - Springer
Cryopreservation of testicular cells and tissues is useful for the preservation and restoration
of fertility in pre-pubertal males expecting gonadotoxic treatment for cancer and genetic …

Solid surface vitrification of goat testicular cell suspension enriched for spermatogonial stem cells

T Patra, MK Gupta - Cryobiology, 2022 - Elsevier
This study reports solid surface vitrification (SSV) of goat testicular cell suspensions (TCS)
enriched for spermatogonial stem cells (SSCs). The TCS was isolated from pre-pubertal …

Comparison of two culture methods during in vitro spermatogenesis of vitrified-warmed testis tissue: Organ culture vs. hanging drop culture

T Patra, D Pathak, MK Gupta - Cryobiology, 2021 - Elsevier
Solid surface vitrification (SSV) is a cost effective and simple method for testis tissue
preservation. Vitrified-warmed testis tissue was successfully cultured using various organ …

Tracking immature testicular tissue after vitrification in vitro and in vivo for pre-pubertal fertility preservation: a translational transgenic mouse model

BJ Lu, YL Huang, YL Liu, BS Chen, BZ Lin… - International journal of …, 2022 - mdpi.com
Pediatric cancer survivors experiencing gonadotoxic chemoradiation therapy may encounter
subfertility or permanent infertility. However, previous studies of cryopreservation of …

Droplet Generation, Vitrification, and Warming for Cell Cryopreservation: A Review

M Cui, T Zhan, J Yang, H Dang, G Yang… - ACS Biomaterials …, 2023 - ACS Publications
Cryopreservation is currently a key step in translational medicine that could provide new
ideas for clinical applications in reproductive medicine, regenerative medicine, and cell …

Effect of Different Vitrification Techniques on Viability and Apoptotic Index of Domestic Cat Testicular Tissue Cells

JVG Carvalho, ARB Soares, DL Leão, AN Reis… - Animals, 2023 - mdpi.com
Simple Summary The vitrification process stands out among cryopreservation methods
because it is a practical and fast technique that reduces the risk of intracellular ice formation …