Hydrogen exchange coupled to mass spectrometry (MS) has become a valuable analytical
tool for the study of protein dynamics. By combining information about protein dynamics with …

The regulated dephosphorylation of mitogen-activated protein kinases (MAPKs) plays a key
role in determining the magnitude and duration of kinase activation and hence the …

Solution-phase hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) is
a widespread tool for structural analysis across academia and the biopharmaceutical …

A wide range of regulatory processes in the cell are mediated by flexible peptides that fold
upon binding to globular proteins. Computational efforts to model these interactions are …

Post-translational modifications (PTMs) can have profound effects on protein structure and
protein dynamics and thereby can influence protein function. To understand and connect …

Flexible peptides that fold upon binding to another protein molecule mediate a large number
of regulatory interactions in the living cell and may provide highly specific recognition …

Protein phosphorylation, first elucidated as a regulatory mechanism in 1955, 1 is very likely
the most important mechanism for regulation in mammalian cells. While allosteric regulation …

▪ Abstract Mass spectrometry has provided a powerful method for monitoring hydrogen
exchange of protein backbone amides with deuterium from solvent. In comparison to …

This four-volume laboratory manual contains comprehensive state-of-the-art protocols
essential for research in the life sciences. Techniques are presented in a friendly step-by …

Amide hydrogen/deuterium exchange is a powerful biophysical technique for probing
changes in protein dynamics induced by ligand interaction. The inherent low throughput of …