New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields

L Adler, A Díaz-Ramos, Y Mao, KR Pukacz… - Plant …, 2022 - academic.oup.com
Many photosynthetic species have evolved CO2-concentrating mechanisms (CCMs) to
improve the efficiency of CO2 assimilation by Rubisco and reduce the negative impacts of …

The CO2 concentrating mechanism and photosynthetic carbon assimilation in limiting CO2: how Chlamydomonas works against the gradient

Y Wang, DJ Stessman, MH Spalding - The Plant Journal, 2015 - Wiley Online Library
The CO 2 concentrating mechanism (CCM) represents an effective strategy for carbon
acquisition that enables microalgae to survive and proliferate when the CO 2 concentration …

The eukaryotic CO2-concentrating organelle is liquid-like and exhibits dynamic reorganization

ESF Rosenzweig, B Xu, LK Cuellar… - Cell, 2017 - cell.com
Summary Approximately 30%–40% of global CO 2 fixation occurs inside a non-membrane-
bound organelle called the pyrenoid, which is found within the chloroplasts of most …

Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii

F Fauser, J Vilarrasa-Blasi, M Onishi, S Ramundo… - Nature …, 2022 - nature.com
Most genes in photosynthetic organisms remain functionally uncharacterized. Here, using a
barcoded mutant library of the model eukaryotic alga Chlamydomonas reinhardtii, we …

A spatial interactome reveals the protein organization of the algal CO2-concentrating mechanism

LCM Mackinder, C Chen, RD Leib, W Patena, SR Blum… - Cell, 2017 - cell.com
Approximately one-third of global CO 2 fixation is performed by eukaryotic algae. Nearly all
algae enhance their carbon assimilation by operating a CO 2-concentrating mechanism …

A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle

LCM Mackinder, MT Meyer… - Proceedings of the …, 2016 - National Acad Sciences
Biological carbon fixation is a key step in the global carbon cycle that regulates the
atmosphere's composition while producing the food we eat and the fuels we burn …

The structural basis of Rubisco phase separation in the pyrenoid

S He, HT Chou, D Matthies, T Wunder, MT Meyer… - Nature plants, 2020 - nature.com
Approximately one-third of global CO2 fixation occurs in a phase-separated algal organelle
called the pyrenoid. The existing data suggest that the pyrenoid forms by the phase …

[HTML][HTML] State transitions—the molecular remodeling of photosynthetic supercomplexes that controls energy flow in the chloroplast

J Minagawa - Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2011 - Elsevier
In oxygen-evolving photosynthesis, the two photosystems—photosystem I and photosystem
II—function in parallel, and their excitation levels must be balanced to maintain an optimal …

[HTML][HTML] Pyrenoids: CO2-fixing phase separated liquid organelles

J Barrett, P Girr, LCM Mackinder - … et Biophysica Acta (BBA)-Molecular Cell …, 2021 - Elsevier
Pyrenoids are non-membrane bound organelles found in chloroplasts of algae and hornwort
plants that can be seen by light-microscopy. Pyrenoids are formed by liquid-liquid phase …

The pyrenoid: the eukaryotic CO2-concentrating organelle

S He, VL Crans, MC Jonikas - The Plant Cell, 2023 - academic.oup.com
The pyrenoid is a phase-separated organelle that enhances photosynthetic carbon
assimilation in most eukaryotic algae and the land plant hornwort lineage. Pyrenoids …