▪ Abstract Proteins are designed to function in environments crowded by cosolutes, but most
studies of protein equilibria are conducted in dilute solution. While there is no doubt that …

High protein concentration solutions are becoming increasingly important in the
pharmaceutical industry. The solution behavior of proteins at high concentrations can …

Thermally induced transition curves of hen egg-white lysozyme were measured in the
presence of several concentrations of dextran at pH 2.0 by near-UV and far-UV CD. The …

Cell cytoplasm contains high concentrations of high-molecular-weight components that
occupy a substantial part of the volume of the medium (crowding conditions). The effect of …

Aggregation of proteins, even under conditions favoring the native state, is a ubiquitous
problem in biotechnology and biomedical engineering. Providing a mechanistic basis for the …

Effects of "crowding" in protein solutions Page 1 Effects of “Crowding” in Protein Solutions G. B.
Ralston Department of Biochemistry, University of Sydney, Sydney, NSW, 2006, Australia Most …

Consideration is given to the interpretation of virial coefficients reflecting thermodynamic
nonideality in incompressible solutions of a single macromolecular species for which there …

Given the importance of protein complexes as therapeutic targets, it is necessary to
understand the physical chemistry of these interactions under the crowded conditions that …

Inhibition by compatible solutes such as proline and glycine betaine of the rate of
coagulation, at 60° C, of bovine serum albumin in 0.1 m acetate buffer, pH 5, is used as a …

Cell cytoplasm contains high concentrations of macromolecules occupying a significant part
of the cell volume (crowding conditions). According to modern concepts, crowding has a …