Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges

L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

The emergence of phase separation as an organizing principle in bacteria

CA Azaldegui, AG Vecchiarelli, JS Biteen - Biophysical journal, 2021 - cell.com
Recent investigations in bacteria suggest that membraneless organelles play a crucial role
in the subcellular organization of bacterial cells. However, the biochemical functions and …

Bioinspired ice-binding materials for tissue and organ cryopreservation

Z Liu, X Zheng, J Wang - Journal of the American Chemical …, 2022 - ACS Publications
Cryopreservation of tissues and organs can bring transformative changes to medicine and
medical science. In the past decades, limited progress has been achieved, although …

Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy

J Kwon, MS Elgawish, SH Shim - Advanced Science, 2022 - Wiley Online Library
Photobleaching is the permanent loss of fluorescence after extended exposure to light and
is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal …

Liquid-liquid phase separation: Unraveling the enigma of biomolecular condensates in microbial cells

Z Gao, W Zhang, R Chang, S Zhang, G Yang… - Frontiers in …, 2021 - frontiersin.org
Numerous examples of microbial phase-separated biomolecular condensates have now
been identified following advances in fluorescence imaging and single molecule microscopy …

openFrame: A modular, sustainable, open microscopy platform with single‐shot, dual‐axis optical autofocus module providing high precision and long range of …

J Lightley, S Kumar, MQ Lim, E Garcia… - Journal of …, 2023 - Wiley Online Library
Abstract 'openFrame'is a modular, low‐cost, open‐hardware microscopy platform that can
be configured or adapted to most light microscopy techniques and is easily upgradeable or …

Advanced imaging techniques for studying protein phase separation in living cells and at single-molecule level

G Mekonnen, N Djaja, X Yuan, S Myong - Current Opinion in Chemical …, 2023 - Elsevier
Protein-protein and protein-RNA interactions are essential for cell function and survival.
These interactions facilitate the formation of ribonucleoprotein complexes and biomolecular …

Direct-laser writing for subnanometer focusing and single-molecule imaging

S Coelho, J Baek, J Walsh, J Justin Gooding… - Nature …, 2022 - nature.com
Two-photon direct laser writing is an additive fabrication process that utilizes two-photon
absorption of tightly focused femtosecond laser pulses to implement spatially controlled …