Assembly and maintenance of lipids at the bacterial outer membrane

E Lundstedt, D Kahne, N Ruiz - Chemical reviews, 2020 - ACS Publications
The outer membrane of Gram-negative bacteria is essential for their survival in harsh
environments and provides intrinsic resistance to many antibiotics. This membrane is …

The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life

J Beld, EC Sonnenschein, CR Vickery, JP Noel… - Natural product …, 2014 - pubs.rsc.org
Covering: up to 2013 Although holo-acyl carrier protein synthase, AcpS, a
phosphopantetheinyl transferase (PPTase), was characterized in the 1960s, it was not until …

The Essential Genome of Escherichia coli K-12

ECA Goodall, A Robinson, IG Johnston, S Jabbari… - MBio, 2018 - Am Soc Microbiol
Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method
coupling transposon mutagenesis with short-fragment DNA sequencing. It is commonly used …

Structure of the essential inner membrane lipopolysaccharide–PbgA complex

T Clairfeuille, KR Buchholz, Q Li, E Verschueren, P Liu… - Nature, 2020 - nature.com
Lipopolysaccharide (LPS) resides in the outer membrane of Gram-negative bacteria where it
is responsible for barrier function,. LPS can cause death as a result of septic shock, and its …

Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli

S Shu, W Mi - Nature Communications, 2022 - nature.com
Lipopolysaccharide (LPS) is an essential glycolipid and forms a protective permeability
barrier for most Gram-negative bacteria. In E. coli, LPS levels are under feedback control …

Border control: regulating LPS biogenesis

RL Guest, ST Rutherford, TJ Silhavy - Trends in Microbiology, 2021 - cell.com
The outer membrane (OM) is a defining feature of Gram-negative bacteria that serves as a
permeability barrier and provides rigidity to the cell. Critical to OM function is establishing …

YejM modulates activity of the YciM/FtsH protease complex to prevent lethal accumulation of lipopolysaccharide

RL Guest, D Samé Guerra, M Wissler, J Grimm… - MBio, 2020 - Am Soc Microbiol
Lipopolysaccharide (LPS) is an essential glycolipid present in the outer membrane (OM) of
many Gram-negative bacteria. Balanced biosynthesis of LPS is critical for cell viability; too …

Lipid trafficking across the Gram-negative cell envelope

R Shrivastava, SS Chng - Journal of Biological Chemistry, 2019 - ASBMB
The outer membrane (OM) of Gram-negative bacteria exhibits unique lipid asymmetry, with
lipopolysaccharides (LPS) residing in the outer leaflet and phospholipids (PLs) in the inner …

An essential membrane protein modulates the proteolysis of LpxC to control lipopolysaccharide synthesis in Escherichia coli

EM Fivenson, TG Bernhardt - MBio, 2020 - Am Soc Microbiol
Gram-negative bacteria are surrounded by a complex cell envelope that includes two
membranes. The outer membrane prevents many drugs from entering these cells and is thus …

Delivery of cardiolipins to the Salmonella outer membrane is necessary for survival within host tissues and virulence

ZD Dalebroux, MB Edrozo, RA Pfuetzner, S Ressl… - Cell host & …, 2015 - cell.com
The outer membrane (OM) of Gram-negative bacteria is an asymmetric lipid bilayer that
serves as a barrier to the environment. During infection, Gram-negative bacteria remodel …