Advances in the automation of whole-cell patch clamp technology

HJ Suk, ES Boyden, I van Welie - Journal of neuroscience methods, 2019 - Elsevier
Electrophysiology is the study of neural activity in the form of local field potentials, current
flow through ion channels, calcium spikes, back propagating action potentials and somatic …

Progress in automating patch clamp cellular physiology

LA Annecchino, SR Schultz - Brain and neuroscience …, 2018 - journals.sagepub.com
Patch clamp electrophysiology has transformed research in the life sciences over the last
few decades. Since their inception, automatic patch clamp platforms have evolved …

High-throughput microcircuit analysis of individual human brains through next-generation multineuron patch-clamp

Y Peng, FX Mittermaier, H Planert, UC Schneider… - Elife, 2019 - elifesciences.org
Comparing neuronal microcircuits across different brain regions, species and individuals
can reveal common and divergent principles of network computation. Simultaneous patch …

[HTML][HTML] Closed-loop real-time imaging enables fully automated cell-targeted patch-clamp neural recording in vivo

HJ Suk, I van Welie, SB Kodandaramaiah, B Allen… - Neuron, 2017 - cell.com
Targeted patch-clamp recording is a powerful method for characterizing visually identified
cells in intact neural circuits, but it requires skill to perform. We previously developed an …

[HTML][HTML] Automatic deep learning-driven label-free image-guided patch clamp system

K Koos, G Oláh, T Balassa, N Mihut, M Rózsa… - Nature …, 2021 - nature.com
Patch clamp recording of neurons is a labor-intensive and time-consuming procedure. Here,
we demonstrate a tool that fully automatically performs electrophysiological recordings in …

In Vivo Glutamate Sensing inside the Mouse Brain with Perovskite Nickelate–Nafion Heterostructures

Y Sun, TNH Nguyen, A Anderson… - … applied materials & …, 2020 - ACS Publications
Glutamate, one of the main neurotransmitters in the brain, plays a critical role in
communication between neurons, neuronal development, and various neurological …

Visual experience-dependent oscillations and underlying circuit connectivity changes are impaired in Fmr1 KO mice

ST Kissinger, Q Wu, CJ Quinn, AK Anderson, A Pak… - Cell reports, 2020 - cell.com
Fragile X syndrome (FX), the most common inherited form of autism and intellectual
disability, is a condition associated with visual perceptual learning deficits. We recently …

Visual familiarity induced 5-Hz oscillations and improved orientation and direction selectivities in V1

M Gao, S Lim, AA Chubykin - Journal of Neuroscience, 2021 - Soc Neuroscience
Neural oscillations play critical roles in information processing, communication between
brain areas, learning, and memory. We have recently discovered that familiar visual stimuli …

PatcherBot: a single-cell electrophysiology robot for adherent cells and brain slices

I Kolb, CR Landry, MC Yip, CF Lewallen… - Journal of neural …, 2019 - iopscience.iop.org
Objective. Intracellular patch-clamp electrophysiology, one of the most ubiquitous, high-
fidelity techniques in biophysics, remains laborious and low-throughput. While previous …

Deep learning-based real-time detection of neurons in brain slices for in vitro physiology

MC Yip, MM Gonzalez, CR Valenta, MJM Rowan… - Scientific reports, 2021 - nature.com
A common electrophysiology technique used in neuroscience is patch clamp: a method in
which a glass pipette electrode facilitates single cell electrical recordings from neurons …