Thermal proteome profiling (TPP) is based on the principle that, when subjected to heat,
proteins denature and become insoluble. Proteins can change their thermal stability upon …

Phenotype-based screening has emerged as an alternative route for discovering new
chemical entities toward first-in-class therapeutics. However, clarifying their mode of action …

Mass spectrometry (MS) has emerged at the forefront of quantitative proteomic techniques.
Liquid chromatography‐mass spectrometry (LC‐MS) can be used to determine abundances …

Due to their eukaryotic heritage, the differences between a fungal pathogen's molecular
makeup and its human host are small. Therefore, the discovery and subsequent …

Background Monitoring target engagement at various stages of drug development is
essential for natural product (NP)-based drug discovery and development. The cellular …

Artemisin combination therapy (ACT) is the main treatment option for malaria, which is
caused by the intracellular parasite Plasmodium. However, increased resistance to ACT …

Emergence and spread of resistance in Plasmodium falciparum to the frontline treatment
artemisinin-based combination therapies (ACTs) in the epicenter of multidrug resistance of …

Recrudescent infections with the human malaria parasite, Plasmodium falciparum,
presented traditionally the major setback of artemisinin-based monotherapies. Although the …

Nearly all FDA approved drugs and bioactive small molecules exert their effects by binding
to and modulating proteins. Consequently, understanding how small molecules interact with …

Drug target deconvolution can accelerate the drug discovery process by identifying a drug's
targets (facilitating medicinal chemistry efforts) and off-targets (anticipating toxicity effects or …