Background Low-temperature expression of recombinant proteins may be advantageous to
support their proper folding and preserve bioactivity. The generation of expression vectors …

Continuous cultivation of mammalian cells originated in chemostats in the 1950s and,
through a series of technological advancements, has evolved into modern‐day high‐density …

We describe scalable and cost‐efficient production of full length, His‐tagged severe acute
respiratory syndrome coronavirus 2 (SARS‐CoV‐2) spike glycoprotein trimer by Chinese …

Expression of recombinant genes in HEK293 cells is frequently utilized for production of
recombinant proteins and viral vectors. These systems frequently employ the …

Expression of recombinant proteins in mammalian cell factories relies on synthetic
assemblies of genetic parts to optimally control flux through the product biosynthetic …

To successfully engineer mammalian cells for a desired purpose, multiple recombinant
genes are required to be coexpressed at a specific and optimal ratio. In this study, we …

Site‐specific integration (SSI) technology has emerged as an effective approach by the
pharmaceutical industry for the development of recombinant Chinese hamster ovary (CHO) …

For the industrial production of recombinant proteins in mammalian cell lines, a high rate of
gene expression is desired. Therefore, strong viral promoters are commonly used. However …

Lower cultivation temperature dramatically affects cell growth and cellular productivity in
Chinese hamster ovary cells (CHO) and is often used in industrial applications with the aim …

Age‐related macular degeneration (AMD) associated with dysfunction of retinal pigment
epithelial (RPE) cells is the most common cause of untreatable blindness. To advance gene …