Maize is the principal grain crop of the world. It is also the crop where genetic engineering
has been employed to a great extent to improve its various traits. The ability to transform …

Marker genes (MGs) are essential tools for plant research and biotechnology. Positive
selectable marker genes (SMGs) are used in genetic transformation to allow only transgenic …

Switchgrass (Panicum virgatum L.) is a C4 perennial grass and has been identified as a
potential bioenergy crop for cellulosic ethanol because of its rapid growth rate, nutrient use …

Transgene copy number is an important criterion for determining the utility of transgenic
events. Single copy integration events are highly desirable when the objective is to produce …

Key message A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP
expression analysis, and successfully applied to the expression of a selectable marker gene …

The potential of using vector-free minimal gene cassettes (MGCs) with a double terminator
for the enhancement and stabilization of transgene expression was tested in sugarcane …

Sugarcane mosaic virus (SCMV) is a serious disease of monocotyledonous plants, including
sugarcane, causing deterioration in both growth and productivity. RNA interference (RNAi) …

The influence of two binary vector systems, pGreen and pCAMBIA, on the Agrobacterium-
mediated transformation ability of wheat and triticale was studied. Both vectors carried …

While being one of the world's most important crops, maize (Zea mays L.) is still relatively
difficult to regenerate in tissue culture, which severely limits its improvement by genetic …

The use of particle gun for the production of marker-free plants is scant in published
literature. Perhaps this is a reflection of the widely held notion that the events generated …