Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Elimination or mitigation of the toxic effects of chemical waste released to the environment
by industrial and urban activities relies largely on the catalytic activities of microorganisms …

Abstract Rhea (https://www. rhea-db. org) is an expert-curated knowledgebase of
biochemical reactions based on the chemical ontology ChEBI (Chemical Entities of …

Synthetic biology applied to industrial biotechnology is transforming the way we produce
chemicals. However, despite advances in the scale and scope of metabolic engineering, the …

Engineered biological systems such as genetic circuits and microbial cell factories have
promised to solve many challenges in the modern society. However, the artisanal processes …

Low yield and low titer of natural products are common issues in natural product
biosynthesis through microbial cell factories. One effective way to resolve such bottlenecks …

Metabolic engineering aims to produce chemicals of interest from living organisms, to
advance toward greener chemistry. Despite efforts, the research and development process …

Cell-free transcription–translation systems have great potential for biosensing, yet the range
of detectable chemicals is limited. Here we provide a workflow to expand the range of …

Allosteric transcription factors (aTFs) have proven widely applicable for biotechnology and
synthetic biology as ligand-specific biosensors enabling real-time monitoring, selection and …

How individual enzymes evolved is relatively well understood. However, individual enzymes
rarely confer a physiological advantage on their own. Judging by its current state, the …