Production of recombinant proteins by yeast cells

E Çelik, P Çalık - Biotechnology advances, 2012 - Elsevier
Yeasts are widely used in production of recombinant proteins of medical or industrial
interest. For each individual product, the most suitable expression system has to be …

The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing

T Maniatis, R Reed - Nature, 1987 - nature.com
A small set of distinctive short RNA molecules are found in the nuclei of all higher eukaryotic
cells and yeast, in protein complexes known as' small nuclear ribonucleoprotein particles', or …

[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe

S Moreno, A Klar, P Nurse - Methods in enzymology, 1991 - Elsevier
Publisher Summary This chapter describes techniques concerned with classical and
molecular genetics, cell biology, and biochemistry that can be used with …

A novel nuclear structure containing the survival of motor neurons protein.

Q Liu, G Dreyfuss - The EMBO journal, 1996 - embopress.org
Spinal muscular atrophy (SMA) is a common, often fatal, autosomal recessive disease
leading to progressive muscle wasting and paralysis as a result of degeneration of anterior …

[HTML][HTML] nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.

K Maundrell - Journal of Biological Chemistry, 1990 - Elsevier
The first fully repressible gene in fission yeast is described. In minimal medium it is highly
transcribed producing a mRNA which is 50-100 times more abundant than the cyc1 mRNA …

In vivo and in vitro arginine methylation of RNA-binding proteins

Q Liu, G Dreyfuss - Molecular and cellular biology, 1995 - Am Soc Microbiol
Heterogenous nuclear ribonucleoproteins (hnRNPs) bind pre-mRNAs and facilitate their
processing into mRNAs. Many of the hnRNPs undergo extensive posttranslational …

High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe

K Okazaki, N Okazaki, K Kume, S Jinno… - Nucleic acids …, 1990 - academic.oup.com
We describe a highly efficient alkali cation method and library transducing vectors for
cloning mammalian cDNAs by trans-complementation of fission yeast Schizosaccharomyces …

Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development.

A Sugimoto, Y Iino, T Maeda, Y Watanabe… - Genes & …, 1991 - genesdev.cshlp.org
Schizosaccharomyces pombe ste11 encodes a member of the family of HMG-box proteins.
Its transcript is induced in response to nitrogen starvation and a concomitant decrease of the …

Splicing of messenger RNA precursors

PA Sharp - Science, 1987 - science.org
A general mechanism for the splicing of nuclear messenger RNA precursors in eukaryotic
cells has been widely accepted. This mechanism, which generates lariat RNAs possessing …

The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation

V Simanis, P Nurse - Cell, 1986 - cell.com
The cdc2+ gene function has an important role in controlling the commitment of the fission
yeast cell to the mitotic cycle and the timing of mitosis. We have raised antibodies against …