Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

The proliferation of microscopy methods for live-cell imaging offers many new possibilities
for users but can also be challenging to navigate. The prevailing challenge in live-cell …

A common goal of fluorescence microscopy is to collect data on specific biological events.
Yet, the event-specific content that can be collected from a sample is limited, especially for …

This article reviews deep learning applications in biomedical optics with a particular
emphasis on image formation. The review is organized by imaging domains within …

Propelled by the striking advances in optical microscopy and deep learning (DL), the role of
imaging in lab-on-a-chip has dramatically been transformed from a silo inspection tool to a …

Optical super-resolution microscopy (SRM) has enabled biologists to visualize cellular
structures with near-molecular resolution, giving unprecedented access to details about the …

Multi-emitter localization has great potential for maximizing the imaging speed of super-
resolution localization microscopy. However, the slow image analysis speed of reported …

Single-molecule localization microscopy enables three-dimensional fluorescence imaging
at tens-of-nanometer resolution, but requires many camera frames to reconstruct a super …