Abstract Filter Aided Sample Preparation (FASP) is a widely used protein processing
technique in “bottom-up” proteomics. Its popularity reflects the key features of the method: its …

Liquid chromatography coupled to tandem mass spectrometry (LC‐MS/MS) is now the main
analytical method for the identification and quantification of peptides and proteins in …

Robust, efficient, and reproducible protein extraction and sample processing is a key step for
bottom-up proteomics analyses. While many sample preparation protocols for mass …

The incorporation of machine learning methods into proteomics workflows improves the
identification of disease-relevant biomarkers and biological pathways. However, machine …

The main challenge of bottom-up proteomic sample preparation is to extract proteomes in a
manner that enables efficient protein digestion for subsequent mass spectrometric analysis …

Phosphoproteomics can provide insights into cellular signaling dynamics. To achieve deep
and robust quantitative phosphoproteomics profiling for minute amounts of sample, we here …

Alzheimer's disease (AD) is characterized by accumulation of misfolded proteins. Genetic
studies implicate microglia, brain-resident phagocytic immune cells, in AD pathogenesis. As …

Environmental toxicology and ecotoxicology research efforts are employing proteomics with
fish models as New Approach Methodologies, along with in silico, in vitro and other omics …

Selecting a sample preparation strategy for mass spectrometry-based proteomics is critical
to the success of quantitative workflows. Here we present a universal, solid-phase protein …

Tissues and biofluids are important sources of information used for the detection of diseases
and decisions on patient therapies. There are several accepted methods for preservation of …