How human TET2 enzyme catalyzes the oxidation of unnatural cytosine modifications in double-stranded DNA

SO Waheed, A Varghese, SS Chaturvedi… - ACS …, 2022 - ACS Publications
Methylation of cytosine bases is strongly linked to gene expression, imprinting, aging, and
carcinogenesis. The ten-eleven translocation (TET) family of enzymes, which are Fe (II)/2 …

Computational investigations of selected enzymes from two iron and α-ketoglutarate-dependent families

MB Berger, AR Walker… - Physical Chemistry …, 2021 - pubs.rsc.org
DNA alkylation is used as the key epigenetic mark in eukaryotes, however, most alkylation in
DNA can result in deleterious effects. Therefore, this process needs to be tightly regulated …

The pH‐Dependence of the Hydration of 5‐Formylcytosine: an Experimental and Theoretical Study

FL Zott, V Korotenko, H Zipse - ChemBioChem, 2022 - Wiley Online Library
Formylcytosine is an important nucleobase in epigenetic regulation, whose hydrate form has
been implicated in the formation of 5‐carboxycytosine as well as oligonucleotide binding …

A Chemical Model of a TET Enzyme for Selective Oxidation of Hydroxymethyl Cytosine to Formyl Cytosine

D Palit, S Kundu, PK Pain, R Sarma… - Inorganic Chemistry, 2023 - ACS Publications
Methylation/demethylation of cytosines in DNA is central to epigenetics, which plays crucial
roles in the regulation of about half of all human genes. Although the methylation …

Selective and catalytic conversion of hydroxymethyl cytosine into formyl cytosine using a synthetic model of TET enzymes

D Palit, D Manna - Inorganic Chemistry Frontiers, 2024 - pubs.rsc.org
TET enzymes, known as the ten–eleven translocation enzymes, have become central
figures in epigenetic regulation due to their remarkable ability to oxidize 5-methylcytosine (5 …

A high-throughput effector screen identifies a novel small molecule scaffold for inhibition of ten-eleven translocation dioxygenase 2

S Palei, J Weisner, M Vogt, R Gontla… - RSC Medicinal …, 2022 - pubs.rsc.org
Ten-eleven translocation dioxygenases (TETs) are the erasers of 5-methylcytosine (mC), the
central epigenetic regulator of mammalian DNA. TETs convert mC to three oxidized …

Development of a rapid mass spectrometric method for the analysis of ten-eleven translocation enzymes

C Graves, K Islam - Mononuclear Non-heme Iron Dependent …, 2024 - books.google.com
1. Introduction 2. Preparation of the materials 2.1 Expression and purification of wild type
TET2 2.2 Mutagenesis and expression of V1395A 2.3 Synthesis and characterization of …