Conformational dynamics of intrinsically disordered proteins regulate biomolecular condensate chemistry
Motions in biomolecules are critical for biochemical reactions. In cells, many biochemical
reactions are executed inside of biomolecular condensates formed by ultradynamic …
reactions are executed inside of biomolecular condensates formed by ultradynamic …
Fluorescence correlation spectroscopy
J Ries, P Schwille - BioEssays, 2012 - Wiley Online Library
Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure
concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to …
concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to …
[图书][B] Handbook of fluorescence spectroscopy and imaging: from ensemble to single molecules
Providing much-needed information on fluorescence spectroscopy and microscopy, this
ready reference covers detection techniques, data registration, and the use of spectroscopic …
ready reference covers detection techniques, data registration, and the use of spectroscopic …
Diffusion of nanoparticles in a biofilm
TO Peulen, KJ Wilkinson - Environmental science & technology, 2011 - ACS Publications
In order to evaluate the risk of engineered nanomaterials in the natural environment, one
must determine their mobility, among other factors. Such determinations are difficult given …
must determine their mobility, among other factors. Such determinations are difficult given …
Bacterial Hsp70 resolves misfolded states and accelerates productive folding of a multi-domain protein
The ATP-dependent Hsp70 chaperones (DnaK in E. coli) mediate protein folding in
cooperation with J proteins and nucleotide exchange factors (E. coli DnaJ and GrpE …
cooperation with J proteins and nucleotide exchange factors (E. coli DnaJ and GrpE …
[HTML][HTML] Precise measurement of diffusion coefficients using scanning fluorescence correlation spectroscopy
Z Petrášek, P Schwille - Biophysical journal, 2008 - cell.com
We have implemented scanning fluorescence correlation spectroscopy (sFCS) for precise
determination of diffusion coefficients of fluorescent molecules in solution. The measurement …
determination of diffusion coefficients of fluorescent molecules in solution. The measurement …
Quantifying protein–protein interactions by molecular counting with mass photometry
F Soltermann, EDB Foley, V Pagnoni… - Angewandte Chemie …, 2020 - Wiley Online Library
Interactions between biomolecules control the processes of life in health and their
malfunction in disease, making their characterization and quantification essential …
malfunction in disease, making their characterization and quantification essential …
Strongly emissive individual DNA-encapsulated Ag nanoclusters as single-molecule fluorophores
T Vosch, Y Antoku, JC Hsiang… - Proceedings of the …, 2007 - National Acad Sciences
The water-soluble, near-IR-emitting DNA-encapsulated silver nanocluster presented herein
exhibits extremely bright and photostable emission on the single-molecule and bulk levels …
exhibits extremely bright and photostable emission on the single-molecule and bulk levels …
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
[HTML][HTML] Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy
R Macháň, M Hof - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2010 - Elsevier
Investigation of lipid lateral mobility in biological membranes and their artificial models
provides information on membrane dynamics and structure; methods based on optical …
provides information on membrane dynamics and structure; methods based on optical …