Conformational dynamics of intrinsically disordered proteins regulate biomolecular condensate chemistry

A Abyzov, M Blackledge, M Zweckstetter - Chemical Reviews, 2022 - ACS Publications
Motions in biomolecules are critical for biochemical reactions. In cells, many biochemical
reactions are executed inside of biomolecular condensates formed by ultradynamic …

Fluorescence correlation spectroscopy

J Ries, P Schwille - BioEssays, 2012 - Wiley Online Library
Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure
concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to …

[图书][B] Handbook of fluorescence spectroscopy and imaging: from ensemble to single molecules

M Sauer, J Hofkens, J Enderlein - 2010 - books.google.com
Providing much-needed information on fluorescence spectroscopy and microscopy, this
ready reference covers detection techniques, data registration, and the use of spectroscopic …

Diffusion of nanoparticles in a biofilm

TO Peulen, KJ Wilkinson - Environmental science & technology, 2011 - ACS Publications
In order to evaluate the risk of engineered nanomaterials in the natural environment, one
must determine their mobility, among other factors. Such determinations are difficult given …

Bacterial Hsp70 resolves misfolded states and accelerates productive folding of a multi-domain protein

R Imamoglu, D Balchin, M Hayer-Hartl… - Nature …, 2020 - nature.com
The ATP-dependent Hsp70 chaperones (DnaK in E. coli) mediate protein folding in
cooperation with J proteins and nucleotide exchange factors (E. coli DnaJ and GrpE …

[HTML][HTML] Precise measurement of diffusion coefficients using scanning fluorescence correlation spectroscopy

Z Petrášek, P Schwille - Biophysical journal, 2008 - cell.com
We have implemented scanning fluorescence correlation spectroscopy (sFCS) for precise
determination of diffusion coefficients of fluorescent molecules in solution. The measurement …

Quantifying protein–protein interactions by molecular counting with mass photometry

F Soltermann, EDB Foley, V Pagnoni… - Angewandte Chemie …, 2020 - Wiley Online Library
Interactions between biomolecules control the processes of life in health and their
malfunction in disease, making their characterization and quantification essential …

Strongly emissive individual DNA-encapsulated Ag nanoclusters as single-molecule fluorophores

T Vosch, Y Antoku, JC Hsiang… - Proceedings of the …, 2007 - National Acad Sciences
The water-soluble, near-IR-emitting DNA-encapsulated silver nanocluster presented herein
exhibits extremely bright and photostable emission on the single-molecule and bulk levels …

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

E Lerner, A Barth, J Hendrix, B Ambrose, V Birkedal… - Elife, 2021 - elifesciences.org
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

[HTML][HTML] Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

R Macháň, M Hof - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2010 - Elsevier
Investigation of lipid lateral mobility in biological membranes and their artificial models
provides information on membrane dynamics and structure; methods based on optical …