Open the technical black box of tumor mutational burden (TMB): factors affecting harmonization and standardization of panel-based TMB
MT Sung, YH Wang, CF Li - International Journal of Molecular Sciences, 2022 - mdpi.com
As tumor mutational burden (TMB) has been approved as a predictive biomarker for immune
checkpoint inhibitors (ICIs), next-generation sequencing (NGS) TMB panels are being …
checkpoint inhibitors (ICIs), next-generation sequencing (NGS) TMB panels are being …
Human papillomavirus detection by whole-genome next-generation sequencing: importance of validation and quality assurance procedures
LS Arroyo Mühr, D Guerendiain, K Cuschieri… - Viruses, 2021 - mdpi.com
Next-generation sequencing (NGS) yields powerful opportunities for studying human
papillomavirus (HPV) genomics for applications in epidemiology, public health, and clinical …
papillomavirus (HPV) genomics for applications in epidemiology, public health, and clinical …
Relationship between formalin reagent and success rate of targeted sequencing analysis using formalin fixed paraffin embedded tissues
K Amemiya, Y Hirotsu, T Oyama, M Omata - Clinica Chimica Acta, 2019 - Elsevier
Background Tumor genetic alterations are determined to aid in selecting therapy and
predicting prognosis. In routine clinical practice, targeted sequencing analysis is performed …
predicting prognosis. In routine clinical practice, targeted sequencing analysis is performed …
Cold formalin fixation guarantees DNA integrity in formalin fixed paraffin embedded tissues: premises for a better quality of diagnostic and experimental pathology with …
E Berrino, L Annaratone, U Miglio, E Maldi… - Frontiers in …, 2020 - frontiersin.org
Formalin fixation and paraffin embedding (FFPE) represent the standard method to preserve
tissue specimens for diagnostic pathology, however formalin fixation induces severe …
tissue specimens for diagnostic pathology, however formalin fixation induces severe …
Impact of DNA integrity on the success rate of tissue‐based next‐generation sequencing: Lessons from nationwide cancer genome screening project SCRUM‐Japan …
T Kuwata, M Wakabayashi, Y Hatanaka… - Pathology …, 2020 - Wiley Online Library
In the nationwide cancer genome screening project SCRUM‐Japan GI‐SCREEN, 2590
archival formalin‐fixed paraffin‐embedded (FFPE) tumor tissues from 19 institutions were …
archival formalin‐fixed paraffin‐embedded (FFPE) tumor tissues from 19 institutions were …
Diagnostic utility of DNA integrity number as an indicator of sufficient DNA quality in next-generation sequencing–based genomic profiling
K Hiramatsu, C Matsuda, K Masago… - American Journal of …, 2023 - academic.oup.com
Objectives DNA integrity number (DIN) is a metric for assessing DNA degradation,
calculated based on electrophoresis using the Agilent TapeStation System. The utility of DIN …
calculated based on electrophoresis using the Agilent TapeStation System. The utility of DIN …
Comprehensive development and implementation of good laboratory practice for NGS based targeted panel on solid tumor FFPE tissues in diagnostics
The speed, accuracy, and increasing affordability of next-generation sequencing (NGS)
have revolutionized the advent of precision medicine. To date, standardized validation …
have revolutionized the advent of precision medicine. To date, standardized validation …
Tissue fixation with a formic acid-deprived formalin better preserves DNA integrity over time
E Berrino, L Annaratone, P Detillo, D Grassini… - Pathobiology, 2023 - karger.com
Introduction: Optimization of pre-analytic procedures and tissue processing is a basic
requirement for reliable and reproducible data to be obtained. Tissue fixation in formalin …
requirement for reliable and reproducible data to be obtained. Tissue fixation in formalin …
Heterogeneity of tumour mutational burden in metastatic NSCLC demonstrated by endobronchial ultrasound sampling
Introduction Tumour mutational burden (TMB) is an important emerging biomarker for
immune checkpoint inhibitors (ICI). The stability of TMB values across distinct EBUS tumour …
immune checkpoint inhibitors (ICI). The stability of TMB values across distinct EBUS tumour …
The Water‐Exclusion Trap (WET): A 3D printable window trap collector that prevents DNA degradation
Sampling is one of the most important and fundamental processes in field biology. Among
many sampling methods in entomology, window traps have long been considered one of the …
many sampling methods in entomology, window traps have long been considered one of the …