Development of AI-assisted microscopy frameworks through realistic simulation with pySTED

A Bilodeau, A Michaud-Gagnon, J Chabbert… - Nature Machine …, 2024 - nature.com
The integration of artificial intelligence into microscopy systems significantly enhances
performance, optimizing both image acquisition and analysis phases. Development of …

A NIR-Fluorochrome for Live Cell Dual Emission and Lifetime Tracking from the First Plasma Membrane Interaction to Subcellular and Extracellular Locales

E Booth, M Garre, D Wu, HC Daly, DF O'Shea - Molecules, 2024 - mdpi.com
Molecular probes with the ability to differentiate between subcellular variations in acidity
levels remain important for the investigation of dynamic cellular processes and functions. In …

The Quantitative In Vivo Assessment of Diabetic and Non‐Diabetic Skin Wound Healing Using Phasor‐FLIM Approach

H Zuhayri, TB Lepekhina, VV Nikolaev… - Journal of …, 2024 - Wiley Online Library
ABSTRACT A quantitative assessment of wound status in a murine model was developed
using phasor plot presentation of fluorescence lifetime imaging microscopy (FLIM) data. The …

[PDF][PDF] 快速荧光寿命显微成像技术及其在活体应用的研究进展(特邀)

林方睿, 王义强, 易敏, 张晨爽, 刘丽炜… - Laser & Optoelectronics …, 2024 - researching.cn
摘要荧光寿命显微成像(FLIM) 已经广泛应用于生命科学研究领域, 具有高灵敏和高特异性的特点
, 在对组织微环境进行定量表征方面具有独特优势, 但由于成像速度相对较慢, 限制了FLIM …

Deep learning-based virtual H& E staining from label-free autofluorescence lifetime images

Q Wang, AR Akram, DA Dorward, S Talas, B Monks… - npj Imaging, 2024 - nature.com
Label-free autofluorescence lifetime is a unique feature of the inherent fluorescence signals
emitted by natural fluorophores in biological samples. Fluorescence lifetime imaging …

FLIMPA: A versatile software for Fluorescence Lifetime Imaging Microscopy Phasor Analysis

S Kapsiani, NF Läubli, EN Ward, M Shehata… - bioRxiv, 2024 - biorxiv.org
Fluorescence lifetime imaging microscopy (FLIM) is an advanced microscopy technique
capable of providing a deeper understanding of the molecular environment of a fluorophore …

Improving FLIM Resolution with Mean-Shift Super-Resolution Microscopy, an analytical approach

M González-Gutiérrez, E Torres-García… - … and Analysis (SIPAIM …, 2023 - ieeexplore.ieee.org
Fluorescence Lifetime Imaging Microscopy (FLIM) is utilized to study the spatial distribution
of fluorophores and assess molecular properties within cells or tissues. However, FLIM faces …

Impact of Machine Learning on Raman and Raman Optical Activity (ROA) Spectroscopic Analyses of ribonucleic acid structure

O Azher - Egyptian Academic Journal of Biological Sciences. C …, 2024 - journals.ekb.eg
The potential of machine learning (ML) to revolutionize analytical sciences, especially,
Raman and ROI spectra collection for RNA nucleotides is paramount. ML provides …