Fluorescence recovery after photobleaching in material and life sciences: putting theory into practice

N Lorén, J Hagman, JK Jonasson… - Quarterly reviews of …, 2015 - cambridge.org
Fluorescence recovery after photobleaching (FRAP) is a versatile tool for determining
diffusion and interaction/binding properties in biological and material sciences. An …

Fluorescence recovery after photobleaching in colloidal science: introduction and application

AA Moud - ACS Biomaterials Science & Engineering, 2022 - ACS Publications
FRAP (fluorescence recovery after photo bleaching) is a method for determining diffusion in
material science. In industrial applications such as medications, foods, Medtech, hygiene …

Quantifying dynamics in phase-separated condensates using fluorescence recovery after photobleaching

NO Taylor, MT Wei, HA Stone, CP Brangwynne - Biophysical journal, 2019 - cell.com
Cells contain numerous membraneless organelles that assemble by intracellular liquid-
liquid phase separation. The viscous properties and associated biomolecular mobility within …

Wide-range displacement sensor based on fiber-optic Fabry–Perot interferometer for subnanometer measurement

X Zhou, Q Yu - IEEE sensors journal, 2010 - ieeexplore.ieee.org
A displacement sensor with subnanometer resolution based on the fiber-optic Fabry-Perot
interferometer is proposed. The Fabry-Perot cavity is formed between the fiber end face and …

Protein diffusion in mammalian cell cytoplasm

T Kühn, TO Ihalainen, J Hyväluoma, N Dross… - PloS one, 2011 - journals.plos.org
We introduce a new method for mesoscopic modeling of protein diffusion in an entire cell.
This method is based on the construction of a three-dimensional digital model cell from …

Diffusion anisotropy in collagen gels and tumors: the effect of fiber network orientation

T Stylianopoulos, B Diop-Frimpong, LL Munn, RK Jain - Biophysical journal, 2010 - cell.com
The interstitial matrix is comprised of cross-linked collagen fibers, generally arranged in
nonisotropic orientations. Spatial alignment of matrix components within the tissue can affect …

Kinetics of FKBP12. 6 binding to ryanodine receptors in permeabilized cardiac myocytes and effects on Ca sparks

T Guo, RL Cornea, S Huke, E Camors, Y Yang… - Circulation …, 2010 - Am Heart Assoc
Rationale: FK506-binding proteins FKBP12. 6 and FKBP12 are associated with cardiac
ryanodine receptors (RyR2), and cAMP-dependent protein kinase A (PKA)-dependent …

Diffusion coefficients of organic molecules in sucrose–water solutions and comparison with Stokes–Einstein predictions

Y Chenyakin, DA Ullmann, E Evoy… - Atmospheric …, 2017 - acp.copernicus.org
The diffusion coefficients of organic species in secondary organic aerosol (SOA) particles
are needed to predict the growth and reactivity of these particles in the atmosphere …

Sustained oscillations of NF-κB produce distinct genome scanning and gene expression profiles

MH Sung, L Salvatore, R De Lorenzi, A Indrawan… - PloS one, 2009 - journals.plos.org
NF-κB is a prototypic stress-responsive transcription factor that acts within a complex
regulatory network. The signaling dynamics of endogenous NF-κB in single cells remain …

Fluorescence perturbation techniques to study mobility and molecular dynamics of proteins in live cells: FRAP, photoactivation, photoconversion, and FLIP

A Bancaud, S Huet, G Rabut… - Cold Spring Harbor …, 2010 - cshprotocols.cshlp.org
The technique of fluorescence recovery after photobleaching (FRAP) was introduced in the
mid-1970s to study the diffusion of biomolecules in living cells. For several years, it was …