Reassessment of miRNA variant (isomiRs) composition by small RNA sequencing

C Gómez-Martín, E Aparicio-Puerta… - Cell Reports …, 2023 - cell.com
IsomiRs, sequence variants of mature microRNAs, are usually detected and quantified using
high-throughput sequencing. Many examples of their biological relevance have been …

NORMSEQ: a tool for evaluation, selection and visualization of RNA-Seq normalization methods

C Scheepbouwer, M Hackenberg… - Nucleic Acids …, 2023 - academic.oup.com
RNA-sequencing has become one of the most used high-throughput approaches to gain
knowledge about the expression of all different RNA subpopulations. However, technical …

[HTML][HTML] IsoSeek for unbiased and UMI-informed sequencing of miRNAs from low input samples at single-nucleotide resolution

MAJ van Eijndhoven, C Scheepbouwer… - STAR protocols, 2023 - Elsevier
Besides canonical microRNAs (miRNAs), sequence-based variations called isomiRs have
biological relevance and diagnostic potential; however, accurate calling of these post …