Oxford nanopore sequencing in clinical microbiology and infection diagnostics

D Sheka, N Alabi, PMK Gordon - Briefings in bioinformatics, 2021 - academic.oup.com
Extended turnaround times and large economic costs hinder the usage of currently applied
screening methods for bacterial pathogen identification (ID) and antimicrobial susceptibility …

[HTML][HTML] DNA diagnostics for reliable and universal identification of Helicobacter pylori

P Sulo, B Šipková - World Journal of Gastroenterology, 2021 - ncbi.nlm.nih.gov
Reliable diagnostics are a major challenge for the detection and treatment of Helicobacter
pylori (H. pylori) infection. Currently at the forefront are non-invasive urea breath test (UBT) …

[HTML][HTML] Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice

H Xie, J Fang, MA Farag, Z Li, P Sun, P Shao - Food Chemistry: X, 2022 - Elsevier
In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was
used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were …

Gut Bacteroides act in a microbial consortium to cause susceptibility to severe malaria

RK Mandal, A Mandal, JE Denny, R Namazii… - Nature …, 2023 - nature.com
Malaria is caused by Plasmodium species and remains a significant cause of morbidity and
mortality globally. Gut bacteria can influence the severity of malaria, but the contribution of …

Interleukin-22 signaling attenuates necrotizing enterocolitis by promoting epithelial cell regeneration

B Mihi, Q Gong, LS Nolan, SE Gale, M Goree, E Hu… - Cell Reports …, 2021 - cell.com
Necrotizing enterocolitis (NEC) is a deadly intestinal inflammatory disorder that primarily
affects premature infants and lacks adequate therapeutics. Interleukin (IL)-22 plays a critical …

Alterations of the gut microbiota in response to total sleep deprivation and recovery sleep in rats

Z Wang, K Yuan, YB Ji, SX Li, L Shi… - Nature and Science …, 2022 - Taylor & Francis
Introduction Accumulating evidence suggests that both sleep loss and gut dysbiosis can
lead to metabolic disorders. However, less is known about the impact of total sleep …

Rapid and sensitive recombinase polymerase amplification combined with lateral flow strips for detecting Candida albicans

F Wang, D Ge, L Wang, N Li, H Chen, Z Zhang… - Analytical …, 2021 - Elsevier
Owing to modern lifestyles and increasing amounts of medical intervention, clinical
infections caused by conditionally pathogenic fungi are becoming increasingly serious …

Sex-dependent effects of intestinal microbiome manipulation in a mouse model of Alzheimer's disease

H Kaur, S Nookala, S Singh, S Mukundan… - Cells, 2021 - mdpi.com
Mechanisms linking intestinal bacteria and neurodegenerative diseases such as
Alzheimer's disease (AD) are still unclear. We hypothesized that intestinal dysbiosis might …

Prospective comparison between shotgun metagenomics and sanger sequencing of the 16S rRNA gene for the etiological diagnosis of infections

C Lamoureux, L Surgers, V Fihman, G Gricourt… - Frontiers in …, 2022 - frontiersin.org
Bacteriological diagnosis is traditionally based on culture. However, this method may be
limited by the difficulty of cultivating certain species or by prior exposure to antibiotics, which …

Diagnostic reliability of nested PCR depends on the primer design and threshold abundance of Helicobacter pylori in biopsy, stool, and saliva samples

B Šeligová, Ľ Lukáč, M Bábelová, S Vávrová… - Helicobacter, 2020 - Wiley Online Library
Background The aim of this work was to find a reliable nested PCR for the detection of
Helicobacter pylori in biopsy, stool, and saliva specimens. Materials and Methods Novel …