Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Artificial metalloenzymes: reaction scope and optimization strategies
The incorporation of a synthetic, catalytically competent metallocofactor into a protein
scaffold to generate an artificial metalloenzyme (ArM) has been explored since the late …
scaffold to generate an artificial metalloenzyme (ArM) has been explored since the late …
The medicinal chemist's toolbox for late stage functionalization of drug-like molecules
T Cernak, KD Dykstra, S Tyagarajan… - Chemical Society …, 2016 - pubs.rsc.org
The advent of modern C–H functionalization chemistries has enabled medicinal chemists to
consider a synthetic strategy, late stage functionalization (LSF), which utilizes the C–H …
consider a synthetic strategy, late stage functionalization (LSF), which utilizes the C–H …
Advances in ultrahigh-throughput screening for directed enzyme evolution
U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
Directed evolution of artificial metalloenzymes for in vivo metathesis
The field of biocatalysis has advanced from harnessing natural enzymes to using directed
evolution to obtain new biocatalysts with tailor-made functions. Several tools have recently …
evolution to obtain new biocatalysts with tailor-made functions. Several tools have recently …
Abiological catalysis by artificial haem proteins containing noble metals in place of iron
HM Key, P Dydio, DS Clark, JF Hartwig - Nature, 2016 - nature.com
Enzymes that contain metal ions—that is, metalloenzymes—possess the reactivity of a
transition metal centre and the potential of molecular evolution to modulate the reactivity and …
transition metal centre and the potential of molecular evolution to modulate the reactivity and …
Constructing biocatalytic cascades: in vitro and in vivo approaches to de novo multi-enzyme pathways
The combination of sequential biocatalytic reactions, via non-natural synthetic cascades, is a
rapidly developing field and leads to the generation of complex valuable chemicals from …
rapidly developing field and leads to the generation of complex valuable chemicals from …
Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet
The two main strategies for enzyme engineering, directed evolution and rational design,
have found widespread applications in improving the intrinsic activities of proteins. Although …
have found widespread applications in improving the intrinsic activities of proteins. Although …
Noncovalent interactions in metal complex catalysis
KT Mahmudov, AV Gurbanov, FI Guseinov… - Coordination Chemistry …, 2019 - Elsevier
Metal complex catalysis, in which coordination compounds are designed as catalysts for
chemical transformations employing noncovalent interactions, is emerging as a powerful …
chemical transformations employing noncovalent interactions, is emerging as a powerful …
An artificial metalloenzyme with the kinetics of native enzymes
P Dydio, HM Key, A Nazarenko, JYE Rha… - Science, 2016 - science.org
Natural enzymes contain highly evolved active sites that lead to fast rates and high
selectivities. Although artificial metalloenzymes have been developed that catalyze …
selectivities. Although artificial metalloenzymes have been developed that catalyze …