In vivo medical imaging has made great progress due to advances in the engineering of
imaging devices and developments in the chemistry of imaging probes. Several modalities …

The combination of fluorescent‐probe technology plus modern optical microscopes allows
investigators to monitor dynamic events in living cells with exquisite temporal and spatial …

Luminescence lifetime‐based sensing is ideally suited to monitor biological systems due to
its minimal invasiveness and remote working principle. Yet, its applicability is limited in …

Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for
quantitative imaging, enabling precise measurement of cellular metabolites, molecular …

Actomyosin stress fibers (SFs) enable cells to exert traction on planar extracellular matrices
(ECMs) by tensing focal adhesions (FAs) at the cell–ECM interface. Although it is widely …

Fluorescence lifetime imaging microscopy (FLIM) is a method for measuring fluorophore
lifetimes with microscopic spatial resolution, providing a useful tool for cell biologists to …

We present a new high-speed lifetime measurement scheme of analog mean-delay (AMD)
method which is suitable for studying dynamical time-resolved spectroscopy and high-speed …

The fluorescence resonant energy transfer (FRET) from a donor to an acceptor via transition
dipole–dipole interactions decreases the donor's fluorescent lifetime. The donor's …

In biological applications of fluorescence lifetime imaging, low signals from samples can be
a challenge, causing poor lifetime precision. We demonstrate how optimal signal gating (a …

Förster Resonance Energy Transfer (FRET) enables the observation of interactions at the
nanoscale level through the use of fluorescence optical imaging techniques. In FRET …