The crucial role of methodology development in directed evolution of selective enzymes
Directed evolution of stereo‐, regio‐, and chemoselective enzymes constitutes a unique way
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …
to generate biocatalysts for synthetically interesting transformations in organic chemistry and …
Utility of B-factors in protein science: interpreting rigidity, flexibility, and internal motion and engineering thermostability
Z Sun, Q Liu, G Qu, Y Feng, MT Reetz - Chemical reviews, 2019 - ACS Publications
The term B-factor, sometimes called the Debye–Waller factor, temperature factor, or atomic
displacement parameter, is used in protein crystallography to describe the attenuation of X …
displacement parameter, is used in protein crystallography to describe the attenuation of X …
Role of conformational dynamics in the evolution of novel enzyme function
MA Maria-Solano, E Serrano-Hervás… - Chemical …, 2018 - pubs.rsc.org
The free energy landscape concept that describes enzymes as an ensemble of differently
populated conformational sub-states in dynamic equilibrium is key for evaluating enzyme …
populated conformational sub-states in dynamic equilibrium is key for evaluating enzyme …
Unlocking the stereoselectivity and substrate acceptance of enzymes: Proline‐induced loop engineering test
G Qu, Y Bi, B Liu, J Li, X Han, W Liu… - Angewandte Chemie …, 2022 - Wiley Online Library
Protein stability and evolvability influence each other. Although protein dynamics play
essential roles in various catalytically important properties, their high flexibility and diversity …
essential roles in various catalytically important properties, their high flexibility and diversity …
Development of an engineered ketoreductase with simultaneously improved thermostability and activity for making a bulky atorvastatin precursor
XM Gong, Z Qin, FL Li, BB Zeng, GW Zheng… - ACS Catalysis, 2018 - ACS Publications
Protein engineering is a powerful strategy for enhancing the properties of enzymes for
industrial applications. However, thermostabilizing an enzyme via this strategy while …
industrial applications. However, thermostabilizing an enzyme via this strategy while …
Current trends in protein engineering: updates and progress
R Sinha, P Shukla - Current Protein and Peptide Science, 2019 - ingentaconnect.com
Proteins are one of the most important and resourceful biomolecules that find applications in
health, industry, medicine, research, and biotechnology. Given its tremendous relevance …
health, industry, medicine, research, and biotechnology. Given its tremendous relevance …
Efficient reductive desymmetrization of bulky 1, 3-cyclodiketones enabled by structure-guided directed evolution of a carbonyl reductase
X Chen, H Zhang, MA Maria-Solano, W Liu, J Li… - Nature Catalysis, 2019 - nature.com
Abstract Reductive desymmetrization of 2, 2-disubstituted prochiral 1, 3-cyclodiketones to 2,
2-disubstituted-3-hydroxycycloketones is a highly desired transformation for the construction …
2-disubstituted-3-hydroxycycloketones is a highly desired transformation for the construction …
Conformational dynamics‐guided loop engineering of an alcohol dehydrogenase: capture, turnover and enantioselective transformation of difficult‐to‐reduce ketones
B Liu, G Qu, JK Li, W Fan, JA Ma, Y Xu… - … Synthesis & Catalysis, 2019 - Wiley Online Library
Directed evolution of enzymes for the asymmetric reduction of prochiral ketones to produce
enantio‐pure secondary alcohols is particularly attractive in organic synthesis. Loops …
enantio‐pure secondary alcohols is particularly attractive in organic synthesis. Loops …
Engineering the catalytic activity of an Antarctic PET‐degrading enzyme by loop exchange
P Blázquez‐Sánchez, JA Vargas, AA Furtado… - Protein …, 2023 - Wiley Online Library
Several hydrolases have been described to degrade polyethylene terephthalate (PET) at
moderate temperatures ranging from 25° C to 40° C. These mesophilic PET hydrolases …
moderate temperatures ranging from 25° C to 40° C. These mesophilic PET hydrolases …
KnowVolution of a GH5 Cellulase from Penicillium verruculosum to Improve Thermal Stability for Biomass Degradation
F Contreras, MJ Thiele, S Pramanik… - ACS Sustainable …, 2020 - ACS Publications
Understanding the thermostability of cellulases is of high importance for their application in
lignocellulosic biomass degradation, feedstock, and pulp and paper production. Cellulases …
lignocellulosic biomass degradation, feedstock, and pulp and paper production. Cellulases …