Synthetic microbiology in sustainability applications

EM Jones, JP Marken, PA Silver - Nature Reviews Microbiology, 2024 - nature.com
Microorganisms are a promising means to address many societal sustainability challenges
owing to their ability to thrive in diverse environments and interface with the microscale …

[HTML][HTML] The life and death of RNA across temperatures

A Becskei, S Rahaman - Computational and structural biotechnology …, 2022 - Elsevier
Temperature is an environmental condition that has a pervasive effect on cells along with all
the molecules and reactions in them. The mechanisms by which prototypical RNA molecules …

Split complementation of base editors to minimize off-target edits

X Xiong, K Liu, Z Li, FN Xia, XM Ruan, X He, JF Li - Nature Plants, 2023 - nature.com
Base editors (BEs) empower the efficient installation of beneficial or corrective point
mutations in crop and human genomes. However, conventional BEs can induce …

A pathway independent multi-modular ordered control system based on thermosensors and CRISPRi improves bioproduction in Bacillus subtilis

W Yu, K Jin, Y Wu, Q Zhang, Y Liu, J Li… - Nucleic Acids …, 2022 - academic.oup.com
Dynamic regulation is an effective strategy for control of gene expression in microbial cell
factories. In some pathway contexts, several metabolic modules must be controlled in a time …

Developing a dynamic equilibrium system in Escherichia coli to improve the production of recombinant proteins

ZX Zhang, YZ Wang, FT Nong, Y Xu, C Ye, Y Gu… - Applied Microbiology …, 2022 - Springer
The combination of Escherichia coli BL21 (DE3) and the pET expression system is used
extensively for the expression of various recombinant proteins (RPs). However, RP …

Programming mammalian cell behaviors by physical cues

J Huang, M Fussenegger - Trends in Biotechnology, 2024 - cell.com
In recent decades, the field of synthetic biology has witnessed remarkable progress, driving
advances in both research and practical applications. One pivotal area of development …

Expanding the genetic programmability of Lactiplantibacillus plantarum

M Blanch‐Asensio, S Dey, VS Tadimarri… - Microbial …, 2024 - Wiley Online Library
Lactobacilli are ubiquitous in nature and symbiotically provide health benefits for countless
organisms including humans, animals and plants. They are vital for the fermented food …

A novel heat-inducible plasmid-driven T7 system for enhancing carbonic anhydrase in engineered Escherichia coli strains

RE Hu, WC Leu, YC Lin, IS Ng - Process Biochemistry, 2024 - Elsevier
Over the past decades, the heat-inducible (HI) promoter, derived from cI857 phage system
has been widely utilized. However, the developments of alternative temperature-controlled …

1Progress, applications, challenges and prospects of protein purification technology

M Du, Z Hou, L Liu, Y Xuan, X Chen, L Fan… - … in Bioengineering and …, 2022 - frontiersin.org
Protein is one of the most important biological macromolecules in life, which plays a vital
role in cell growth, development, movement, heredity, reproduction and other life activities …

Phytosensors: harnessing plants to understand the world around us

AC Pfotenhauer, SC Lenaghan - Current Opinion in Biotechnology, 2024 - Elsevier
Highlights•Review of the state of the art approaches for current phytosensor
designs.•Analysis of the future design space for phytosensors.•Comparison of strengths and …