Synthetic microbiology in sustainability applications
Microorganisms are a promising means to address many societal sustainability challenges
owing to their ability to thrive in diverse environments and interface with the microscale …
owing to their ability to thrive in diverse environments and interface with the microscale …
[HTML][HTML] The life and death of RNA across temperatures
Temperature is an environmental condition that has a pervasive effect on cells along with all
the molecules and reactions in them. The mechanisms by which prototypical RNA molecules …
the molecules and reactions in them. The mechanisms by which prototypical RNA molecules …
Split complementation of base editors to minimize off-target edits
X Xiong, K Liu, Z Li, FN Xia, XM Ruan, X He, JF Li - Nature Plants, 2023 - nature.com
Base editors (BEs) empower the efficient installation of beneficial or corrective point
mutations in crop and human genomes. However, conventional BEs can induce …
mutations in crop and human genomes. However, conventional BEs can induce …
A pathway independent multi-modular ordered control system based on thermosensors and CRISPRi improves bioproduction in Bacillus subtilis
W Yu, K Jin, Y Wu, Q Zhang, Y Liu, J Li… - Nucleic Acids …, 2022 - academic.oup.com
Dynamic regulation is an effective strategy for control of gene expression in microbial cell
factories. In some pathway contexts, several metabolic modules must be controlled in a time …
factories. In some pathway contexts, several metabolic modules must be controlled in a time …
Developing a dynamic equilibrium system in Escherichia coli to improve the production of recombinant proteins
ZX Zhang, YZ Wang, FT Nong, Y Xu, C Ye, Y Gu… - Applied Microbiology …, 2022 - Springer
The combination of Escherichia coli BL21 (DE3) and the pET expression system is used
extensively for the expression of various recombinant proteins (RPs). However, RP …
extensively for the expression of various recombinant proteins (RPs). However, RP …
Programming mammalian cell behaviors by physical cues
J Huang, M Fussenegger - Trends in Biotechnology, 2024 - cell.com
In recent decades, the field of synthetic biology has witnessed remarkable progress, driving
advances in both research and practical applications. One pivotal area of development …
advances in both research and practical applications. One pivotal area of development …
Expanding the genetic programmability of Lactiplantibacillus plantarum
Lactobacilli are ubiquitous in nature and symbiotically provide health benefits for countless
organisms including humans, animals and plants. They are vital for the fermented food …
organisms including humans, animals and plants. They are vital for the fermented food …
A novel heat-inducible plasmid-driven T7 system for enhancing carbonic anhydrase in engineered Escherichia coli strains
Over the past decades, the heat-inducible (HI) promoter, derived from cI857 phage system
has been widely utilized. However, the developments of alternative temperature-controlled …
has been widely utilized. However, the developments of alternative temperature-controlled …
1Progress, applications, challenges and prospects of protein purification technology
M Du, Z Hou, L Liu, Y Xuan, X Chen, L Fan… - … in Bioengineering and …, 2022 - frontiersin.org
Protein is one of the most important biological macromolecules in life, which plays a vital
role in cell growth, development, movement, heredity, reproduction and other life activities …
role in cell growth, development, movement, heredity, reproduction and other life activities …
Phytosensors: harnessing plants to understand the world around us
AC Pfotenhauer, SC Lenaghan - Current Opinion in Biotechnology, 2024 - Elsevier
Highlights•Review of the state of the art approaches for current phytosensor
designs.•Analysis of the future design space for phytosensors.•Comparison of strengths and …
designs.•Analysis of the future design space for phytosensors.•Comparison of strengths and …