Biointerface design for vertical nanoprobes

R Elnathan, MG Barbato, X Guo, A Mariano… - Nature Reviews …, 2022 - nature.com
Biointerfaces mediate safe and efficient cell manipulation, which is essential for biomedical
innovations in advanced therapies and diagnostics. The biointerface established by vertical …

Recent fabrication methods to produce polymer-based drug delivery matrices (Experimental and In Silico Approaches)

A Procopio, E Lagreca, R Jamaledin, S La Manna… - Pharmaceutics, 2022 - mdpi.com
The study of novel drug delivery systems represents one of the frontiers of the biomedical
research area. Multi-disciplinary scientific approaches combining traditional or engineered …

Engineering Efficient CAR‐T Cells via Electroactive Nanoinjection

AR Shokouhi, Y Chen, HZ Yoh, J Brenker… - Advanced …, 2023 - Wiley Online Library
Chimeric antigen receptor (CAR)‐T cell therapy has emerged as a promising cell‐based
immunotherapy approach for treating blood disorders and cancers, but genetically …

CRISPR/CAS-Assisted Nanoneedle sensor for adenosine triphosphate detection in living cells

H Kim, C Gu, SA Mustfa, DA Martella… - … Applied Materials & …, 2023 - ACS Publications
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein
(Cas)(CRISPR/Cas) systems have recently emerged as powerful molecular biosensing tools …

Electroactive nanoinjection platform for intracellular delivery and gene silencing

AR Shokouhi, Y Chen, HZ Yoh, T Murayama… - Journal of …, 2023 - Springer
Background Nanoinjection—the process of intracellular delivery using vertically configured
nanostructures—is a physical route that efficiently negotiates the plasma membrane, with …

Deep Learning‐Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard‐to‐Transfect Cells

P Mukherjee, CA Patino, N Pathak, V Lemaitre… - Small, 2022 - Wiley Online Library
Genome engineering of cells using CRISPR/Cas systems has opened new avenues for
pharmacological screening and investigating the molecular mechanisms of disease. A …

Role of actin cytoskeleton in cargo delivery mediated by vertically aligned silicon nanotubes

Y Chen, HZ Yoh, AR Shokouhi, T Murayama… - Journal of …, 2022 - Springer
Nanofabrication technologies have been recently applied to the development of engineered
nano–bio interfaces for manipulating complex cellular processes. In particular, vertically …

[HTML][HTML] Single cell transcriptomics reveals reduced stress response in stem cells manipulated using localized electric fields

P Mukherjee, CY Peng, T McGuire, JW Hwang… - Materials Today Bio, 2023 - Elsevier
Abstract Membrane disruption using Bulk Electroporation (BEP) is a widely used non-viral
method for delivering biomolecules into cells. Recently, its microfluidic counterpart …

Changing fate: reprogramming cells via Engineered Nanoscale Delivery materials

S Soltani Dehnavi, Z Eivazi Zadeh… - Advanced …, 2022 - Wiley Online Library
The incorporation of nanotechnology in regenerative medicine is at the nexus of
fundamental innovations and early‐stage breakthroughs, enabling exciting biomedical …

Integrating micro and nano technologies for cell engineering and analysis: toward the next generation of cell therapy workflows

P Mukherjee, SH Park, N Pathak, CA Patino, G Bao… - ACS …, 2022 - ACS Publications
The emerging field of cell therapy offers the potential to treat and even cure a diverse array
of diseases for which existing interventions are inadequate. Recent advances in micro and …