Biocatalysis has become an important aspect of modern organic synthesis, both in
academia and across the chemical and pharmaceutical industries. Its success has been …

Directed evolution has emerged as a powerful strategy to engineer various properties of
proteins. Traditional methods to construct libraries such as error-prone PCR and DNA …

Ancestral sequence reconstruction (ASR) has been used to produce enzymes that exhibit
enhanced stability, activity, and promiscuity compared to wild-type (WT) enzymes. In this …

Enzymes have high catalytic efficiency and low environmental impact, and are therefore
potentially useful tools for various industrial processes. Crucially, however, natural enzymes …

The Trp metabolite l-kynurenine (KYN) accumulates in numerous solid tumours and
mediates potent immunosuppression. Bacterial kynureninases (KYNases), which …

A protein's sequence determines its conformational energy landscape. This, in turn,
determines the protein's function. Understanding the evolution of new protein functions …

Recent years have seen an explosion of interest in understanding the physicochemical
parameters that shape enzyme evolution, as well as substantial advances in computational …

The rapid growth of sequence databases over the past two decades means that protein
engineers faced with optimizing a protein for any given task will often have immediate …

Fatty acid-derived products such as alkanes, fatty aldehydes, and fatty alcohols have many
applications in the chemical industry. These products are predominately produced from …

Carboxylate reductases (CARs) are valuable catalysts for the selective one‐step reduction
of carboxylic acids to their corresponding aldehydes. In recent years, numerous new CARs …