Mass spectrometry (MS) has become one of the key technologies of structural biology. In this
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …

Biological mass spectrometry (MS) encompasses a range of methods for characterizing
proteins and other biomolecules. MS is uniquely powerful for the structural analysis of …

Found in all organisms, pyruvate dehydrogenase complexes (PDC) are the keystones of
prokaryotic and eukaryotic energy metabolism. In eukaryotic organisms these multi …

Found across all kingdoms of life, 2-keto acid dehydrogenase complexes possess
prominent metabolic roles and form major regulatory sites. Although their component …

Cellular function is underlined by megadalton assemblies organizing in proximity, forming
communities. Metabolons are protein communities involving metabolic pathways such as …

The design of new therapeutic molecules can be significantly informed by studying protein-
ligand interactions using biophysical approaches directly after purification of the protein …

Cryo-electron microscopy (cryo-EM) has become an unrivaled tool for determining the
structure of macromolecular complexes. The biological function of macromolecular …

Enzymes that use thiamin diphosphate (ThDP), the biologically active derivative of vitamin
B1, as a cofactor play important roles in cellular metabolism in all domains of life. The …

The Escherichia coli pyruvate dehydrogenase complex (PDHc) is a~ 5 MDa assembly of the
catalytic subunits pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) …

Actinobacteria possess unique ways to regulate the oxoglutarate metabolic node. Contrary
to most organisms in which three enzymes compose the 2-oxoglutarate dehydrogenase …