Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

In industrial bioprocesses, microbial metabolism dictates the product yields, and therefore,
our capacity to control it has an enormous potential to help us move towards a bio-based …

CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

To further increase the production efficiency of microbial shikimate, a valuable compound
widely used in the pharmaceutical and chemical industries, ten key target genes contributing …

Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently
produced from CO 2—but formatotrophy has been engineered in only a few industrially …

Biosensors are used to detect and quantify chemicals produced in industrial microbiology
with high specificity, sensitivity, and portability. Most biosensors, however, are limited by the …

Polyhydroxyalkanoates (PHAs) are promising biopolymers for biomedical applications due
to their excellent biocompatibility and biodegradability. However, the high production cost …

Central carbon metabolism (CCM), including glycolysis, tricarboxylic acid cycle and the
pentose phosphate pathway, is the most fundamental metabolic process in the activities of …

Acetyl-coenzyme A (AcCoA) is a metabolic hub in virtually all living cells, serving as both a
key precursor of essential biomass components and a metabolic sink for catabolic pathways …

Engineering metabolism to efficiently produce chemicals from multi-step pathways requires
optimizing multi-gene expression programs to achieve enzyme balance. CRISPR-Cas …