A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria
Finding a highly sensitive diagnostic technique for malaria has challenged scientists for the
last century. In the present study, we identified versatile single-strand DNA aptamers for …
last century. In the present study, we identified versatile single-strand DNA aptamers for …
[HTML][HTML] Characterization and multi-step transketolase-ω-transaminase bioconversions in an immobilized enzyme microreactor (IEMR) with packed tube
AA Halim, N Szita, F Baganz - Journal of biotechnology, 2013 - Elsevier
The concept of de novo metabolic engineering through novel synthetic pathways offers new
directions for multi-step enzymatic synthesis of complex molecules. This has been …
directions for multi-step enzymatic synthesis of complex molecules. This has been …
[HTML][HTML] Comparative study of fungal cell disruption—scope and limitations of the methods
M Klimek-Ochab, M Brzezińska-Rodak… - Folia microbiologica, 2011 - Springer
Simple and effective protocols of cell wall disruption were elaborated for tested fungal
strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis. Several techniques …
strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis. Several techniques …
Specific enzyme immobilization approaches and their application with nanomaterials
Nanotechnology-inspired biocatalysts have aroused much interest recently because
nanomaterials can provide the upper limits on enzyme-efficiency-determining factors. Non …
nanomaterials can provide the upper limits on enzyme-efficiency-determining factors. Non …
Enhanced protein stability through minimally invasive, direct, covalent, and site‐specific immobilization
Bioconjugating protein to nonbiological surfaces is an essential component of many
promising biotechnologies impacting diverse applications such as medical diagnostics …
promising biotechnologies impacting diverse applications such as medical diagnostics …
[HTML][HTML] Directed evolution of Rhodotorula gracilis d-amino acid oxidase using single-cell hydrogel encapsulation and ultrahigh-throughput screening
Engineering catalytic and biophysical properties of enzymes is an essential step en route to
advanced biomedical and industrial applications. Here, we developed a high-throughput …
advanced biomedical and industrial applications. Here, we developed a high-throughput …
Enhancement in the catalytic efficiency of D-amino acid oxidase from Glutamicibacter protophormiae by multiple amino acid substitutions
S Xu, M Chu, F Zhang, J Zhao, J Zhang, Y Cao… - Enzyme and Microbial …, 2023 - Elsevier
D-Amino acid oxidase (DAAO) is an imperative oxidoreductase that oxidizes D-amino acids
to corresponding keto acids, producing ammonia and hydrogen peroxide. Previously, based …
to corresponding keto acids, producing ammonia and hydrogen peroxide. Previously, based …
[HTML][HTML] CLytA-DAAO chimeric enzyme bound to magnetic nanoparticles. A new therapeutical approach for cancer patients?
M Fuentes-Baile, E Pérez-Valenciano… - International journal of …, 2021 - mdpi.com
D-amino acid oxidase (DAAO) is an enzyme that catalyzes the oxidation of D-amino acids
generating H2O2. The enzymatic chimera formed by DAAO bound to the choline-binding …
generating H2O2. The enzymatic chimera formed by DAAO bound to the choline-binding …
[HTML][HTML] Co-immobilization of D-amino acid oxidase, catalase, and transketolase for one-pot, two-step synthesis of L-erythrulose
D Świętochowska, A Łochowicz, N Ocal, L Pollegioni… - Catalysts, 2023 - mdpi.com
Here, we present an immobilized enzyme cascade in a basket-type reactor allowing a one-
pot, two-step enzymatic synthesis of L-erythrulose from D-serine and glycolaldehyde. Three …
pot, two-step enzymatic synthesis of L-erythrulose from D-serine and glycolaldehyde. Three …
Improving Pullulanase Catalysis via Reversible Immobilization on Modified Fe3O4@Polydopamine Nanoparticles
J Wang, Z Liu, Z Zhou - Applied biochemistry and biotechnology, 2017 - Springer
To improve the catalysis of pullulanase from Anoxybacillus sp. WB42, Fe 3 O 4@
polydopamine nanoparticles (Fe 3 O 4@ PDA) were prepared and modified with functional …
polydopamine nanoparticles (Fe 3 O 4@ PDA) were prepared and modified with functional …