A highly sensitive aptasensor towards Plasmodium lactate dehydrogenase for the diagnosis of malaria

S Lee, KM Song, W Jeon, H Jo, YB Shim… - Biosensors and …, 2012 - Elsevier
Finding a highly sensitive diagnostic technique for malaria has challenged scientists for the
last century. In the present study, we identified versatile single-strand DNA aptamers for …

[HTML][HTML] Characterization and multi-step transketolase-ω-transaminase bioconversions in an immobilized enzyme microreactor (IEMR) with packed tube

AA Halim, N Szita, F Baganz - Journal of biotechnology, 2013 - Elsevier
The concept of de novo metabolic engineering through novel synthetic pathways offers new
directions for multi-step enzymatic synthesis of complex molecules. This has been …

[HTML][HTML] Comparative study of fungal cell disruption—scope and limitations of the methods

M Klimek-Ochab, M Brzezińska-Rodak… - Folia microbiologica, 2011 - Springer
Simple and effective protocols of cell wall disruption were elaborated for tested fungal
strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis. Several techniques …

Specific enzyme immobilization approaches and their application with nanomaterials

W Liu, L Wang, R Jiang - Topics in Catalysis, 2012 - Springer
Nanotechnology-inspired biocatalysts have aroused much interest recently because
nanomaterials can provide the upper limits on enzyme-efficiency-determining factors. Non …

Enhanced protein stability through minimally invasive, direct, covalent, and site‐specific immobilization

MT Smith, JC Wu, CT Varner… - Biotechnology …, 2013 - Wiley Online Library
Bioconjugating protein to nonbiological surfaces is an essential component of many
promising biotechnologies impacting diverse applications such as medical diagnostics …

[HTML][HTML] Directed evolution of Rhodotorula gracilis d-amino acid oxidase using single-cell hydrogel encapsulation and ultrahigh-throughput screening

C Küng, R Vanella, MA Nash - Reaction Chemistry & Engineering, 2023 - pubs.rsc.org
Engineering catalytic and biophysical properties of enzymes is an essential step en route to
advanced biomedical and industrial applications. Here, we developed a high-throughput …

Enhancement in the catalytic efficiency of D-amino acid oxidase from Glutamicibacter protophormiae by multiple amino acid substitutions

S Xu, M Chu, F Zhang, J Zhao, J Zhang, Y Cao… - Enzyme and Microbial …, 2023 - Elsevier
D-Amino acid oxidase (DAAO) is an imperative oxidoreductase that oxidizes D-amino acids
to corresponding keto acids, producing ammonia and hydrogen peroxide. Previously, based …

[HTML][HTML] CLytA-DAAO chimeric enzyme bound to magnetic nanoparticles. A new therapeutical approach for cancer patients?

M Fuentes-Baile, E Pérez-Valenciano… - International journal of …, 2021 - mdpi.com
D-amino acid oxidase (DAAO) is an enzyme that catalyzes the oxidation of D-amino acids
generating H2O2. The enzymatic chimera formed by DAAO bound to the choline-binding …

[HTML][HTML] Co-immobilization of D-amino acid oxidase, catalase, and transketolase for one-pot, two-step synthesis of L-erythrulose

D Świętochowska, A Łochowicz, N Ocal, L Pollegioni… - Catalysts, 2023 - mdpi.com
Here, we present an immobilized enzyme cascade in a basket-type reactor allowing a one-
pot, two-step enzymatic synthesis of L-erythrulose from D-serine and glycolaldehyde. Three …

Improving Pullulanase Catalysis via Reversible Immobilization on Modified Fe3O4@Polydopamine Nanoparticles

J Wang, Z Liu, Z Zhou - Applied biochemistry and biotechnology, 2017 - Springer
To improve the catalysis of pullulanase from Anoxybacillus sp. WB42, Fe 3 O 4@
polydopamine nanoparticles (Fe 3 O 4@ PDA) were prepared and modified with functional …