ThT 101: a primer on the use of thioflavin T to investigate amyloid formation

K Gade Malmos, LM Blancas-Mejia, B Weber… - Amyloid, 2017 - Taylor & Francis
Thioflavin T (ThT) has been widely used to investigate amyloid formation since 1989. While
concerns have recently been raised about its use as a probe specific for amyloid, ThT still …

Molecular mechanism of Thioflavin-T binding to amyloid fibrils

M Biancalana, S Koide - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2010 - Elsevier
Intense efforts to detect, diagnose, and analyze the kinetic and structural properties of
amyloid fibrils have generated a powerful toolkit of amyloid-specific molecular probes. Since …

Thioflavin T as an amyloid dye: fibril quantification, optimal concentration and effect on aggregation

C Xue, TY Lin, D Chang, Z Guo - Royal Society open …, 2017 - royalsocietypublishing.org
Formation of amyloid fibrils underlies a wide range of human disorders, including
Alzheimer's and prion diseases. The amyloid fibrils can be readily detected thanks to …

Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status

M Groenning - Journal of chemical biology, 2010 - Springer
Because understanding amyloid fibrillation in molecular detail is essential for development
of strategies to control amyloid formation and overcome neurodegenerative disorders …

Protein-induced photophysical changes to the amyloid indicator dye thioflavin T

LS Wolfe, MF Calabrese, A Nath… - Proceedings of the …, 2010 - National Acad Sciences
The small molecule thioflavin T (ThT) is a defining probe for the identification and
mechanistic study of amyloid fiber formation. As such, ThT is fundamental to investigations of …

Molecular mechanism of thioflavin-T binding to the surface of β-rich peptide self-assemblies

M Biancalana, K Makabe, A Koide, S Koide - Journal of molecular biology, 2009 - Elsevier
A number of small organic molecules have been developed that bind to amyloid fibrils, a
subset of which also inhibit fibrillization. Among these, the benzothiol dye Thioflavin-T (ThT) …

Revisiting thioflavin T (ThT) fluorescence as a marker of protein fibrillation–The prominent role of electrostatic interactions

E Arad, H Green, R Jelinek, H Rapaport - Journal of colloid and interface …, 2020 - Elsevier
Thioflavin T (ThT), a benzothiazole-based fluorophore, is a prominent dye widely employed
for monitoring amyloid fibril assembly. Despite the near-universal presumption that ThT …

The binding of thioflavin T and its neutral analog BTA-1 to protofibrils of the Alzheimer's disease Aβ16–22 peptide probed by molecular dynamics simulations

C Wu, Z Wang, H Lei, Y Duan, MT Bowers… - Journal of molecular …, 2008 - Elsevier
Thioflavin T (ThT) is a fluorescent dye commonly used to stain amyloid plaques, but the
binding sites of this dye onto fibrils are poorly characterized. We present molecular …

[HTML][HTML] Steady-state and time-resolved Thioflavin-T fluorescence can report on morphological differences in amyloid fibrils formed by Aβ (1-40) and Aβ (1-42)

DJ Lindberg, MS Wranne, MG Gatty… - Biochemical and …, 2015 - Elsevier
Thioflavin-T (ThT) is one of the most commonly used dyes for amyloid detection, but the
origin of its fluorescence enhancement is not fully understood. Herein we have …

Cooperative Metal Ion Coordination to the Short Self‐Assembling Peptide Promotes Hydrogelation and Cellular Proliferation

VK Pal, S Roy - Macromolecular Bioscience, 2022 - Wiley Online Library
Noncovalent interactions among short peptides and proteins lead to their molecular self‐
assembly into supramolecular packaging, which provides the fundamental basis of life …