ThT 101: a primer on the use of thioflavin T to investigate amyloid formation
K Gade Malmos, LM Blancas-Mejia, B Weber… - Amyloid, 2017 - Taylor & Francis
Thioflavin T (ThT) has been widely used to investigate amyloid formation since 1989. While
concerns have recently been raised about its use as a probe specific for amyloid, ThT still …
concerns have recently been raised about its use as a probe specific for amyloid, ThT still …
Molecular mechanism of Thioflavin-T binding to amyloid fibrils
M Biancalana, S Koide - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2010 - Elsevier
Intense efforts to detect, diagnose, and analyze the kinetic and structural properties of
amyloid fibrils have generated a powerful toolkit of amyloid-specific molecular probes. Since …
amyloid fibrils have generated a powerful toolkit of amyloid-specific molecular probes. Since …
Thioflavin T as an amyloid dye: fibril quantification, optimal concentration and effect on aggregation
C Xue, TY Lin, D Chang, Z Guo - Royal Society open …, 2017 - royalsocietypublishing.org
Formation of amyloid fibrils underlies a wide range of human disorders, including
Alzheimer's and prion diseases. The amyloid fibrils can be readily detected thanks to …
Alzheimer's and prion diseases. The amyloid fibrils can be readily detected thanks to …
Binding mode of Thioflavin T and other molecular probes in the context of amyloid fibrils—current status
M Groenning - Journal of chemical biology, 2010 - Springer
Because understanding amyloid fibrillation in molecular detail is essential for development
of strategies to control amyloid formation and overcome neurodegenerative disorders …
of strategies to control amyloid formation and overcome neurodegenerative disorders …
Protein-induced photophysical changes to the amyloid indicator dye thioflavin T
LS Wolfe, MF Calabrese, A Nath… - Proceedings of the …, 2010 - National Acad Sciences
The small molecule thioflavin T (ThT) is a defining probe for the identification and
mechanistic study of amyloid fiber formation. As such, ThT is fundamental to investigations of …
mechanistic study of amyloid fiber formation. As such, ThT is fundamental to investigations of …
Molecular mechanism of thioflavin-T binding to the surface of β-rich peptide self-assemblies
A number of small organic molecules have been developed that bind to amyloid fibrils, a
subset of which also inhibit fibrillization. Among these, the benzothiol dye Thioflavin-T (ThT) …
subset of which also inhibit fibrillization. Among these, the benzothiol dye Thioflavin-T (ThT) …
Revisiting thioflavin T (ThT) fluorescence as a marker of protein fibrillation–The prominent role of electrostatic interactions
Thioflavin T (ThT), a benzothiazole-based fluorophore, is a prominent dye widely employed
for monitoring amyloid fibril assembly. Despite the near-universal presumption that ThT …
for monitoring amyloid fibril assembly. Despite the near-universal presumption that ThT …
The binding of thioflavin T and its neutral analog BTA-1 to protofibrils of the Alzheimer's disease Aβ16–22 peptide probed by molecular dynamics simulations
Thioflavin T (ThT) is a fluorescent dye commonly used to stain amyloid plaques, but the
binding sites of this dye onto fibrils are poorly characterized. We present molecular …
binding sites of this dye onto fibrils are poorly characterized. We present molecular …
[HTML][HTML] Steady-state and time-resolved Thioflavin-T fluorescence can report on morphological differences in amyloid fibrils formed by Aβ (1-40) and Aβ (1-42)
DJ Lindberg, MS Wranne, MG Gatty… - Biochemical and …, 2015 - Elsevier
Thioflavin-T (ThT) is one of the most commonly used dyes for amyloid detection, but the
origin of its fluorescence enhancement is not fully understood. Herein we have …
origin of its fluorescence enhancement is not fully understood. Herein we have …
Cooperative Metal Ion Coordination to the Short Self‐Assembling Peptide Promotes Hydrogelation and Cellular Proliferation
Noncovalent interactions among short peptides and proteins lead to their molecular self‐
assembly into supramolecular packaging, which provides the fundamental basis of life …
assembly into supramolecular packaging, which provides the fundamental basis of life …