Shiga toxins are virulence factors produced by the bacteria Shigella dysenteriae and certain
strains of Escherichia coli. There is currently no available treatment for disease caused by …

Shiga toxin (Stx)-producing Escherichia coli (STEC) cause an enteric illness that results in a
spectrum of outcomes ranging from asymptomatic carriage to uncomplicated diarrhea …

We describe a novel type of molecule in which single-domain antibodies (sdAbs) isolated
from a naı̈ve llama single domain antibody library are linked to an oligomerization domain …

The pH within individual organelles of the secretory pathway is believed to be an important
determinant of their biosynthetic activity. However, little is known about the determinants and …

The factors contributing to the establishment of the steady state Golgi pH (pH G) were
studied in intact and permeabilized mammalian cells by fluorescence ratio imaging …

The B subunit of verotoxin (VT1B) from enterohemorrhagic Escherichia coli is responsible
for the attachment of the holotoxin to the cell surface, by binding to the glycolipid …

Compared to monovalent carbohydrates, multivalent carbohydrate ligands exhibit
significantly enhanced binding affinities to their interacting proteins. Here, we report …

The pentameric B subunit of verotoxin (VT) mediates the attachment to cell surface
globotriaosyl ceramide (Gb3) to facilitate receptor‐mediated endocytosis of the toxin. In …

Sphingosine N-acyltransferase (ceramide synthase, EC 2.3. 1.24) was solubilized from
bovine liver mitochondrion-rich fraction with n-ocytl β-d-thioglucoside as the detergent and …

The presence of cell surface receptor glycolipid, globotriaosylceramide (Gb), is essential to
confer susceptibility to the E. coli-derived verotoxin (VT). Our earlier studies showed that Gb …