A comparative assessment of conventional and molecular methods, including MinION nanopore sequencing, for surveying water quality

K Acharya, S Khanal, K Pantha, N Amatya… - Scientific reports, 2019 - nature.com
Nucleic acid based techniques, such as quantitative PCR (qPCR) and next generation
sequencing (NGS), provide new insights into microbial water quality, but considerable …

Omics in the detection and identification of biosynthetic pathways related to mycotoxin synthesis

NM González-López, KA Huertas-Ortiz… - Analytical …, 2021 - pubs.rsc.org
Mycotoxins are secondary metabolites that are known to be toxic to humans and animals.
On the other hand, some mycotoxins and their analogues possess antioxidant as well as …

18S rDNA gene metabarcoding of microeukaryotes and epi‐endophytes in the holobiome of seven species of large brown algae

MFM Bjorbækmo, J Brodie, AK Krabberød… - Journal of …, 2023 - Wiley Online Library
Brown algae (Phaeophyceae) are habitat‐forming species in coastal ecosystems and
include kelp forests and seaweed beds that support a wide diversity of marine life. Host …

A step towards validation of high-throughput sequencing for the identification of plant pathogenic oomycetes

AS Espindola, K Cardwell, FN Martin… - …, 2022 - Am Phytopath Society
The advancement in high-throughput sequencing (HTS) technology allows the detection of
pathogens without the need for isolation or template amplification. Plant regulatory agencies …

TASPERT: Target-specific reverse transcript pools to improve HTS plant virus diagnostics

AS Espindola, D Sempertegui-Bayas, DF Bravo-Padilla… - Viruses, 2021 - mdpi.com
High-throughput sequencing (HTS) is becoming the new norm of diagnostics in plant
quarantine settings. HTS can be used to detect, in theory, all pathogens present in any given …

Microbe Finder (MiFi®): Implementation of an Interactive Pathogen Detection Tool in Metagenomic Sequence Data

AS Espindola, KF Cardwell - Plants, 2021 - mdpi.com
Agricultural high throughput diagnostics need to be fast, accurate and have multiplexing
capacity. Metagenomic sequencing is being widely evaluated for plant and animal …

Detection of Phytophthora, Pythium, Globisporangium, Hyaloperonospora, and Plasmopara Species in High-Throughput Sequencing Data by In Silico and In Vitro …

F Proaño-Cuenca, AS Espindola… - PhytoFrontiers …, 2023 - Am Phytopath Society
Species identification of plant pathogenic oomycetes based on morphology is challenging
because of the limited number of variable characters available. Hence, pure culture isolation …

Aspergillus Metabolome Database for Mass Spectrometry Metabolomics

A Gil-De-La-Fuente, M Mamani-Huanca, MC Stroe… - Journal of Fungi, 2021 - mdpi.com
The Aspergillus Metabolome Database is a free online resource to perform metabolite
annotation in mass spectrometry studies devoted to the genus Aspergillus. The database …

Aspergillus flavus NRRL 35739, a Poor Biocontrol Agent, May Have Increased Relative Expression of Stress Response Genes

KK Pennerman, G Yin, JW Bennett, SST Hua - Journal of Fungi, 2019 - mdpi.com
Biocontrol of the mycotoxin aflatoxin utilizes non-aflatoxigenic strains of Aspergillus flavus,
which have variable success rates as biocontrol agents. One non-aflatoxigenic strain, NRRL …

Training, tests, and tech: Deployment of diagnostic tools for biosecurity

CL Harmon, BL Akey, FM Ochoa-Corona… - Tactical Sciences for …, 2022 - igi-global.com
Diagnosis of disease is a process of hypothesis, investigation, and synthesis. Regardless of
whether a human, animal, or plant is afflicted, the process of diagnosis is strikingly similar …