High‐throughput identification and diagnostics of pathogens and pests: overview and practical recommendations

L Tedersoo, R Drenkhan, S Anslan… - Molecular ecology …, 2019 - Wiley Online Library
High‐throughput identification technologies provide efficient tools for understanding the
ecology and functioning of microorganisms. Yet, these methods have been only rarely used …

PacBio metabarcoding of Fungi and other eukaryotes: errors, biases and perspectives

L Tedersoo, A Tooming‐Klunderud… - New Phytologist, 2018 - Wiley Online Library
Second‐generation, high‐throughput sequencing methods have greatly improved our
understanding of the ecology of soil microorganisms, yet the short barcodes (< 500 bp) …

[HTML][HTML] Promising perspectives for detection, identification, and quantification of plant pathogenic fungi and oomycetes through targeting mitochondrial DNA

T Kulik, K Bilska, M Żelechowski - International Journal of Molecular …, 2020 - mdpi.com
Fungi and oomycetes encompass many pathogens affecting crops worldwide. Their effective
control requires screening pathogens across the local and international trade networks …

[HTML][HTML] Dilution of specialist pathogens drives productivity benefits from diversity in plant mixtures

G Wang, HM Burrill, LY Podzikowski… - Nature …, 2023 - nature.com
Productivity benefits from diversity can arise when compatible pathogen hosts are buffered
by unrelated neighbors, diluting pathogen impacts. However, the generality of pathogen …

Relative performance of MinION (Oxford Nanopore Technologies) versus Sequel (Pacific Biosciences) third-generation sequencing instruments in identification of …

K Loit, K Adamson, M Bahram, R Puusepp… - Applied and …, 2019 - Am Soc Microbiol
Culture-based molecular identification methods have revolutionized detection of pathogens,
yet these methods are slow and may yield inconclusive results from environmental …

PipeCraft: Flexible open‐source toolkit for bioinformatics analysis of custom high‐throughput amplicon sequencing data

S Anslan, M Bahram, I Hiiesalu… - Molecular ecology …, 2017 - Wiley Online Library
High‐throughput sequencing methods have become a routine analysis tool in
environmental sciences as well as in public and private sector. These methods provide vast …

Towards PacBio‐based pan‐eukaryote metabarcoding using full‐length ITS sequences

L Tedersoo, S Anslan - Environmental microbiology reports, 2019 - Wiley Online Library
Development of high‐throughput sequencing techniques has greatly benefited our
understanding about microbial ecology, yet the methods producing short reads suffer from …

Environmental DNA assessment of airborne plant and fungal seasonal diversity

E Banchi, CG Ametrano, E Tordoni, D Stanković… - Science of the Total …, 2020 - Elsevier
Environmental DNA (eDNA) metabarcoding and metagenomics analyses can improve
taxonomic resolution in biodiversity studies. Only recently, these techniques have been …

Diversity of Phytophthora, Pythium, and Phytopythium Species in Recycled Irrigation Water in a Container Nursery

NR Redekar, JL Eberhart, JL Parke - Phytobiomes Journal, 2019 - Am Phytopath Society
Recycling of irrigation water increases disease risks due to spread of waterborne oomycete
plant pathogens such as Phytophthora, Pythium, and Phytopythium. A comprehensive …

Root pathogen diversity and composition varies with climate in undisturbed grasslands, but less so in anthropogenically disturbed grasslands

CS Delavaux, JL Schemanski, GL House… - The ISME …, 2021 - academic.oup.com
Soil-borne pathogens structure plant communities, shaping their diversity, and through these
effects may mediate plant responses to climate change and disturbance. Little is known …