Escherichia coli is the most widely used heterologous protein expression system. However,
this system remains a challenge due to the low solubility of proteins, insufficient yield, and …

Structural studies of G protein-coupled receptors (GPCRs) gave insights into molecular
mechanisms of their action and contributed significantly to molecular pharmacology. This is …

Cell-free protein synthesis (CFPS) systems are gaining more importance as universal tools
for basic research, applied sciences, and product development with new technologies …

G protein‐coupled receptors (GPCRs) play crucial roles in sensory, immune, and tumor
metastasis processes, making them valuable targets for pharmacological and sensing …

Canonical integral membrane proteins are attached to lipid bilayers through hydrophobic
transmembrane helices, whose topogenesis requires sophisticated insertion machineries …

Voltage-gated Na+ channels are essential for the functioning of cardiovascular, muscular,
and nervous systems. The α-subunit of eukaryotic Na+ channel consists of~ 2000 amino …

G protein-coupled receptors (GPCRs) are of great pharmaceutical interest and about 35% of
the commercial drugs target these proteins. Still there is huge potential left in finding …

Based on their eminent importance for medical applications, G-protein coupled receptors
are currently amongst the most frequently membrane protein targets analyzed by cell-free …

Objective: To study the transport activity of bacterial rhodopsins, proteoliposomes with the
same orientation of the protein molecules should be obtained. Methods: The genes of fusion …

Xanthorhodopsin (XR) from Salinibacter ruber is a light-driven proton pump containing
retinal and a light-harvesting carotenoid antenna salinixanthin. Previous structure-functional …