Targeted capture in evolutionary and ecological genomics

MR Jones, JM Good - Molecular ecology, 2016 - Wiley Online Library
The rapid expansion of next‐generation sequencing has yielded a powerful array of tools to
address fundamental biological questions at a scale that was inconceivable just a few years …

The utility of single nucleotide polymorphism (SNP) data in phylogenetics

AD Leaché, JR Oaks - Annual Review of Ecology, Evolution …, 2017 - annualreviews.org
Resolving the genealogy of life—the phylogenetic relationships that describe the
evolutionary history of species—remains one of the great challenges of systematic biology …

MitoFinder: Efficient automated large‐scale extraction of mitogenomic data in target enrichment phylogenomics

R Allio, A Schomaker‐Bastos… - Molecular ecology …, 2020 - Wiley Online Library
Thanks to the development of high‐throughput sequencing technologies, target enrichment
sequencing of nuclear ultraconserved DNA elements (UCEs) now allows routine inference …

PHYLUCE is a software package for the analysis of conserved genomic loci

BC Faircloth - Bioinformatics, 2016 - academic.oup.com
Targeted enrichment of conserved and ultraconserved genomic elements allows universal
collection of phylogenomic data from hundreds of species at multiple time scales (< 5 Ma to> …

HybPiper: Extracting coding sequence and introns for phylogenetics from high‐throughput sequencing reads using target enrichment

MG Johnson, EM Gardner, Y Liu… - Applications in plant …, 2016 - Wiley Online Library
Premise of the study: Using sequence data generated via target enrichment for
phylogenetics requires reassembly of high‐throughput sequence reads into loci, presenting …

ASTRAL: genome-scale coalescent-based species tree estimation

S Mirarab, R Reaz, MS Bayzid, T Zimmermann… - …, 2014 - academic.oup.com
Motivation: Species trees provide insight into basic biology, including the mechanisms of
evolution and how it modifies biomolecular function and structure, biodiversity and co …

De novo DNA synthesis using polymerase-nucleotide conjugates

S Palluk, DH Arlow, T De Rond, S Barthel… - Nature …, 2018 - nature.com
Oligonucleotides are almost exclusively synthesized using the nucleoside phosphoramidite
method, even though it is limited to the direct synthesis of∼ 200 mers and produces …

Species delimitation using genome-wide SNP data

AD Leaché, MK Fujita, VN Minin… - Systematic …, 2014 - academic.oup.com
The multispecies coalescent has provided important progress for evolutionary inferences,
including increasing the statistical rigor and objectivity of comparisons among competing …

[HTML][HTML] Adapterama I: universal stubs and primers for 384 unique dual-indexed or 147,456 combinatorially-indexed Illumina libraries (iTru & iNext)

TC Glenn, RA Nilsen, TJ Kieran, JG Sanders… - PeerJ, 2019 - peerj.com
Massively parallel DNA sequencing offers many benefits, but major inhibitory cost factors
include:(1) start-up (ie, purchasing initial reagents and equipment);(2) buyin (ie, getting the …

Unforeseen consequences of excluding missing data from next-generation sequences: simulation study of RAD sequences

H Huang, LL Knowles - Systematic biology, 2016 - academic.oup.com
There is a lack of consensus on how next-generation sequence (NGS) data should be
considered for phylogenetic and phylogeographic estimates, with some studies excluding …