Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Biotechnology …, 2015 - Elsevier
In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …

Potential of different enzyme immobilization strategies to improve enzyme performance

C Garcia‐Galan, Á Berenguer‐Murcia… - Advanced Synthesis …, 2011 - Wiley Online Library
Enzyme biocatalysis plays a very relevant role in the development of many chemical
industries, eg, energy, food or fine chemistry. To achieve this goal, enzyme immobilization is …

Carbohydrate binding modules: biochemical properties and novel applications

O Shoseyov, Z Shani, I Levy - Microbiology and molecular biology …, 2006 - Am Soc Microbiol
Polysaccharide-degrading microorganisms express a repertoire of hydrolytic enzymes that
act in synergy on plant cell wall and other natural polysaccharides to elicit the degradation of …

Current and prospective applications of metal ion–protein binding

EKM Ueda, PW Gout, L Morganti - Journal of chromatography A, 2003 - Elsevier
Since immobilized metal ion affinity chromatography (IMAC) was first introduced, several
variants of this method and many other metal affinity-based techniques have been devised …

Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands

CD Hodneland, YS Lee, DH Min… - Proceedings of the …, 2002 - National Acad Sciences
This paper describes a method for the selective and covalent immobilization of proteins to
surfaces with control over the density and orientation of the protein. The strategy is based on …

[图书][B] Handbook of affinity chromatography

T Kline - 1993 - taylorfrancis.com
Outlining the fundamental principles by which all interactions occur, this reference focuses
on harnessing the biochemistry of bioorganic compounds in order to separate them …

Immobilization of penicillin G acylase: the key to optimum performance

AI Kallenberg, F van Rantwijk… - Advanced Synthesis & …, 2005 - Wiley Online Library
Penicillin G acylase is a major industrial biocatalyst that is used in the enzymatic production
of 20,000 ta− 1 of 6‐aminopenicillanic acid, the industrial β‐lactam intermediate, as well as …

Biosensors: electrochemical devices—general concepts and performance

O Smutok, E Katz - Biosensors, 2022 - mdpi.com
This review provides a general overview of different biosensors, mostly concentrating on
electrochemical analytical devices, while briefly explaining general approaches to various …

Cellulose-binding domains: biotechnological applications

I Levy, O Shoseyov - Biotechnology advances, 2002 - Elsevier
Many researchers have acknowledged the fact that there exists an immense potential for the
application of the cellulose-binding domains (CBDs) in the field of biotechnology. This …

Recent developments in the site‐specific immobilization of proteins onto solid supports

JA Camarero - Peptide Science, 2008 - Wiley Online Library
Immobilization of proteins onto surfaces is of great importance in numerous applications,
including protein analysis, drug screening, and medical diagnostics, among others. The …