A vast evolutionarily transient translatome contributes to phenotype and fitness

A Wacholder, SB Parikh, NC Coelho, O Acar… - Cell systems, 2023 - cell.com
Translation is the process by which ribosomes synthesize proteins. Ribosome profiling
recently revealed that many short sequences previously thought to be noncoding are …

Biological factors and statistical limitations prevent detection of most noncanonical proteins by mass spectrometry

A Wacholder, AR Carvunis - PLoS Biology, 2023 - journals.plos.org
Ribosome profiling experiments indicate pervasive translation of short open reading frames
(ORFs) outside of annotated protein-coding genes. However, shotgun mass spectrometry …

DBP7 and YRF1-6 Are Involved in Cell Sensitivity to LiCl by Regulating the Translation of PGM2 mRNA

SK Jagadeesan, M Al-Gafari, J Wang… - International Journal of …, 2023 - mdpi.com
Lithium chloride (LiCl) has been widely researched and utilized as a therapeutic option for
bipolar disorder (BD). Several pathways, including cell signaling and signal transduction …

Lithium chloride sensitivity in yeast and regulation of translation

M Hajikarimlou, K Hunt, G Kirby, S Takallou… - International Journal of …, 2020 - mdpi.com
For decades, lithium chloride (LiCl) has been used as a treatment option for those living with
bipolar disorder (BD). As a result, many studies have been conducted to examine its mode …

Discovery and identification of genes involved in DNA damage repair in yeast

SK Jagadeesan, T Potter, M Al-Gafari, M Hooshyar… - Gene, 2022 - Elsevier
DNA repair defects are common in tumour cells and can lead to misrepair of double-strand
breaks (DSBs), posing a significant challenge to cellular integrity. The overall mechanisms …

Lithium chloride sensitivity connects the activity of PEX11 and RIM20 to the translation of PGM2 and other mRNAs with structured 5'-UTRs

SK Jagadeesan, M Al-Gafari, M Hajikarimlou… - Molecular and Cellular …, 2022 - Springer
Lithium chloride (LiCl) is a widely used and extensively researched drug for the treatment of
bipolar disorder (BD). As a result, LiCl has been the subject of research studying its toxicity …

Modified Phenol/Chloroform‐Free DNA Isolation from Yeast and Other Fungi by Non‐Enzymatic Salting Out Method

M Bandehpour, N Dehghani, HZ Jaliani… - Current …, 2023 - Wiley Online Library
The most commonly used fungal DNA extraction techniques require enzymatic digestion or
hazardous chemicals. Here, we describe a quick non‐enzymatic salt‐out method that we …

Rare detection of noncanonical proteins in yeast mass spectrometry studies

A Wacholder, AR Carvunis - Biorxiv, 2023 - biorxiv.org
Ribosome profiling experiments indicate pervasive translation of short open reading frames
(ORFs) outside of annotated protein-coding genes. However, shotgun mass spectrometry …

[HTML][HTML] Investigating the Activities of CAF20 and ECM32 in the Regulation of PGM2 mRNA Translation

M Al-gafari, SK Jagadeesan, TDD Kazmirchuk… - Biology, 2024 - mdpi.com
Translation is a fundamental process in biology, and understanding its mechanisms is
crucial to comprehending cellular functions and diseases. The regulation of this process is …

[PDF][PDF] A reference translatome map reveals two modes of protein evolution

A Wacholder12, O Acar123, AR Carvunis12 - 2021 - academia.edu
Ribosome profiling experiments demonstrate widespread translation of eukaryotic genomes
outside of 12 annotated protein-coding genes. However, it is unclear how much of this …