To the Editor—The cloning and subsequent engineering of fluorescent proteins (FPs) has
revolutionized methods in biological research1, 2. There are now thousands of FPs …

Genetically encoded fluorescent biosensors are powerful tools used to track chemical
processes in intact biological systems. However, the development and optimization of …

Highlights•Fluorescent proteins continue to be essential tools in life sciences.•
Phototransformable FPs and biosensors facilitate advanced imaging experiments.• …

Genetically encoded fluorescent biosensors provide an attractive means of measuring
chemical changes in single cells on fast timescales (milliseconds to seconds). While their …

The approximately linear scaling of fluorescence quantum yield (ϕ) with fluorescence
lifetime (τ) in fluorescent proteins (FPs) has inspired engineering of brighter fluorophores …

Fluorescent proteins (FPs) have become ubiquitous tools for biological research and
concomitantly they are intriguing molecules that are amenable to study with a wide range of …

The development of fluorescent proteins (FPs) has revolutionized biological imaging.
FusionRed, a monomeric red FP (RFP), is known for its low cytotoxicity and correct …

Fluorescent proteins (FPs) for bioimaging are typically developed by screening mutant
libraries for clones with improved photophysical properties. This approach has resulted in …

The past decades have witnessed an explosion of de novo protein designs with a
remarkable range of scaffolds. It remains challenging, however, to design catalytic functions …

In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high
intracellular brightness. Here we describe a practical method for rapid optimization of …